Back to results
US 5,532,124 AGranted
Genetically engineered bacteria to identify and produce medically important agents
AssigneeThomas Jefferson University
InventorsTimothy M. Block, Robert H. Grafstrom
Life of this patent
Estimating the term…
Abstract
Microorganisms modified such that their growth in selective media is dependent upon the inhibition of a medically important target function are provided and utilized in methods for the screening of potential medically important compounds.
Claims11
A method of identifying an inhibitor of a viral enzyme comprising:
- (a) providing a microorganism having a first expression vector encoding a viral enzyme and a second expression vector comprising a nucleotide sequence encoding a modified tetracycline resistance protein, said modified tetracycline resistance protein having a function which may be deactivated by said viral enzyme;
- (b) culturing the microorganism in a culture medium in the presence of test compound;
- (c) adding tetracycline to said culture medium;
- and
- (d) determining the activity of the modified tetracycline resistance protein function, thereby identifying an inhibitor of a viral enzyme.
2 more elements
The method of claim 37, wherein the packaging recognition sequence consists essentially of SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.