Cloning and expression of DNA encoding a ripening form of a polypeptide having sulfhydryl oxidase activity
AssigneeUnilever Patent Holdings B.V.
InventorsJan Maat, Wouter Musters, Hein Stam, Peter J. Schaap, Peter J. van de Vonderwoort, Jacob Visser, Johannes M. Verbakel
Life of this patent
Estimating the term…
Abstract
The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ("SOX") activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides is desirable. SOX enzyme may be used for treatment of bakery products or for removal of off-flavour from milk or beer.
Claims6
A recombinant DNA material comprising a nucleotide sequence encoding a ripening form of a polypeptide having sulfhydryl oxidase activity wherein the nucleotide sequence is SEQ ID NO:1 or agenetic variant thereof encoding the same polypeptide.
A nucleotide sequence which hybridizes to SEQ ID NO:1 or a genetic variant thereof encoding the same polypeptide, said hybridization being performed in 6 X SSC, 0.5% SDS, 5 X Denhardt solution, 100 microgram single strand herring sperm DNA at 65.degree. C., followed by a wash step in 1 X SCC, 0.1% SDS at 65.degree. C., said nucleotide sequence encoding a ripening form of a polypeptide having sulfhydryl oxidase activity.
Dependencies are inferred from the claim text.