Tyrosinase-activator protein fusion enzyme
AssigneeBiosource Technologies, Inc.
InventorsGuy della-Cioppa, Monto Kumagai
Life of this patent
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Abstract
The present invention relates to a nucleic acid sequence that encodes a fusion enzyme of tyrosinase and a tyrosinase activator protein. Further, the present invention also relates to the amino acid sequence which is encoded by the nucleic acid sequence of the fusion enzyme. The fusion enzyme may also include a linker positioned between the amino acid sequences of the tyrosinase and the tyrosinase activator protein. Still further, the present invention also relates to a vector useful for introducing the nucleic acid sequence encoding the fusion enzyme into an organism. Still further, the present invention relates to melanins made by the fusion enzyme and a method for making melanins using the fusion enzyme.
Claims57
A nucleic acid sequence encoding a fusion enzyme comprising a nucleic acid sequence encoding for a tyrosinase and a nucleic acid sequence encoding for a tyrosinase activator protein.
A vector for transforming a prokaryotic organism, said vector comprising:
- a nucleic acid sequence encoding for a fusion enzyme, said fusion enzyme comprising an amino acid sequence for a tyrosinase and an amino acid sequence encoding for a tyrosinase activator protein;
- and
- a promoter sequence that regulates the transcription of the fusion enzyme.
A fusion enzyme comprising an amino acid sequence for tyrosinase and an amino acid sequence for a tyrosinase activator protein.
A prokaryotic organism which has been transformed with a vector comprising
- a nucleic acid sequence encoding for a fusion enzyme said fusion enzyme comprising an amino acid sequence for a tyrosinase and an amino acid sequence encoding for a tyrosinase activator protein;
- and
- a promoter sequence that regulates the transcription of fusion enzyme.
A method of producing melanins, comprising the steps of:
- (a) growing a prokaryotic organism which has been transformed with a vector comprising:
- a nucleic acid sequence encoding for a fusion enzyme, said fusion enzyme comprising an amino acid sequence for a tyrosinase and an amino acid sequence encoding for a tyrosinase activator protein;
- and
- a promoter sequence that regulates the transcription of the fusion enzyme;
- and
- (b) isolating the melanin produced.
A nucleic acid sequence encoding a fusion enzyme comprising:
- a nucleic acid sequence encoding for a protein that has cresolase activity and catecholase activity;
- and
- a nucleic acid encoding for a gene product that functions as a tyrosinase activator protein.
An amino acid sequence for a fusion enzyme comprising:
- an amino acid sequence for a protein that has cresolase activity and catecholase activity;
- and
- an amino acid sequence for a protein that has a tyrosinase activator function.
Dependencies are inferred from the claim text.