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US 5,527,899 AGranted
Oligonucleotides with inverted polarity
AssigneeGilead Sciences, Inc.
InventorBrian Froehler
Life of this patent
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Abstract
Oligonucleotides having tandem sequences of inverted polarity, i.e., oligonucleotides comprising regions of the formula: ##STR1## wherein --C-- symbolizes any method of coupling the nucleotide sequence of opposite polarity, are useful for forming an extended triple helix with a double-helical nucleotide duplex. The inverted polarity also stabilizes the single-strand oligonucleotides to exonuclease degradation.
Claims25
A method for binding an oligonucleotide to portions of both strands of a target double-helical polynucleotide duplex comprising the step of:
- contacting said target double-helical polynucleotide duplex with said oligonucleotide so as to form a triplex;
- wherein said oligonucleotide comprises
- a first sequence of nucleotides that forms a triplex with a portion of the first strand of said duplex, covalently coupled to a second sequence of nucleotides having inverted polarity from the first sequence that forms a triplex with a portion of the second strand of said duplex, said second strand portion being proximal to said portion on the first strand in said duplex.
Dependencies are inferred from the claim text.