Brazzein sweetener
AssigneeWisconsin Alumni Research Foundation
InventorsBengt G. Hellekant, Ding Ming
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Abstract
Disclosed herein is a protein sweetener that has been isolated from Pentadiplandra brazzeana Baillon. The sweetener is thermostable, lysine rich, and has a relative lasting taste. Also disclosed is a recombinant host capable of producing the sweetener in large quantities. Compositions of this sweetener with other sweeteners are also disclosed.
Claims5
A sweet protein containing an amino acid sequence according to SEQ ID NO:4 amino acid residues 2-54, wherein the protein has been produced recombinantly and is essentially free of Pentadiplandra brazzeana plant material other than Brazzein.
A DNA sequence according to SEQ ID NO:5 bases 4-162, wherein the DNA sequence is located outside of a Pentadiplandra brazzeana Baillon cell, and the DNA sequence encodes the protein of SEQ ID NO:4 residues 2-54, wherein the DNA sequence is essentially free of Pentadiplandra brazzeana plant material other than Brazzein.
Dependencies are inferred from the claim text.