Detection and therapy of lesions with biotin/avidin conjugates
AssigneeImmunomedics, Inc.
InventorDavid M. Goldenberg
Life of this patent
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Abstract
Methods are provided for detecting and/or treating lesions in a patient. The methods use a targeting composition comprised of a biotin and targeting protein conjugate or an avidin and targeting protein conjugate; optionally, a clearing composition comprised of avidin, when the targeting composition is a biotin conjugate, or biotin, when the targeting composition is a avidin conjugate; a detection or therapeutic composition comprised of a conjugate of avidin or biotin with a targeting protein and detection or therapeutic agent; and, optionally, another detection or therapeutic composition comprised of avidin or biotin conjugated to a detection or therapeutic agent. Compositions and kits useful in the methods are also provided.
Claims48
A method of amplifying the detection or treatment of lesions in a subject, comprising the steps of:
- a) parenterally injecting into said subject a targeting composition comprising a targeting protein that specifically binds to a marker substance produced or associated with said lesion compared to non-lesion sites, covalently conjugated to biotin or avidin, and allowing said targeting composition to preferentially accrete at the targeted lesions;
- b) optionally parenterally injecting into said subject a clearing composition that is capable both of clearing said targeting composition from said non-lesion sites and binding to said targeting composition accreted at said lesion, said clearing composition comprising avidin when said targeting composition contains biotin and comprising biotin when said targeting composition contains avidin;
- c) parenterally injecting into said subject a first detection or therapeutic composition comprising a conjugate composed of covalently linked avidin-targeting protein detection or therapeutic agent, when said targeting composition contains avidin, and composed of covalently linker biotin-targeting protein detection or therapeutic agent when said targeting composition contains biotin, wherein said targeting protein may be the same or different than that of a) above, and allowing said detection or therapeutic composition to preferentially accrete at said lesion;
- d) parenterally injecting into said subject, either prior or subsequent to the composition of c) above, a second detection or therapeutic composition comprising a covalent conjugate of avidin-detection or therapeutic agent when said targeting composition of a) above contains biotin and of biotin-detection, or therapeutic agent when said targeting composition of a) above contains avidin;
- wherein step d) above is optional if a clearing composition has been administered.
Dependencies are inferred from the claim text.