Modified hepatocytes and uses therefor
AssigneesWhitehead Institute for Biomedical Research, Howard Hughes Medical Center
InventorsRichard C. Mulligan, James M. Wilson
Life of this patent
Estimating the term…
Abstract
Genetically engineered or transduced hepatocytes which express genetic material of interest introduced or incorporated into them, as well as methods of producing, transplanting and using the genetically engineered hepatocytes are disclosed. The genetic material of interest can be incorporated through the use of a vector, such as a recombinant retrovirus, which contains the genetic material of interest, or by other means.
Claims5
Transduced mammalian hepatocytes having genetic material of interest stably incorporated therein, the transduced hepatocytes being capable of in vivo expression of the polypeptide or protein encoded by the genetic material of interest wherein said genetic material comprises a retroviral vector lacking a selectable marker and selected from the group consisting of .alpha.-SGC and MFG.
A method of making transduced mammalian hepatocytes which express stably incorporated genetic material lacking a selectable marker and encoding at least one protein of interest or at,least one polypeptide of interest, comprising the steps of:
- a) contacting the hepatocytes in vitro with media containing an infectious recombinant retrovirus having a recombinant genome comprising the "genetic material" for antecedent basis of interest wherein said genetic material comprises a retroviral vector lacking a selectable marker selected from the group consisting of .alpha.-SGC and MFG;
- and
- b) maintaining the hepatocytes and the media containing the infectious recombinant retrovirus under conditions appropriate for infection of the hepatocytes by recombinant retrovirus, thereby producing transduced hepatocytes.
Dependencies are inferred from the claim text.