High pullulan content product, and its preparation and uses
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsYoshihide Ozaki, Tatsuo Nomura, Toshio Miyake
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Abstract
Disclosed is a novel high pullulan content product containing pullulan having an average molecular weight less than 250,000, said product being prepared by continuously cultivating a microorganism capable of producing pullulan in a nutrient culture medium containing a 10-20 w/v % saccharide while controlling the viscosity of the nutrient culture medium to a level below 30 cp. The product can be advantageously used in a variety of fields such as viscosity-imparting agent, coating agent, adhesive, formed product, food product, cosmetic, pharmaceutical, and material for agriculture, forestry, stock raising and paper processings, as well as for mining and manufacturing industries.
Claims12
A process for preparing a pullulan product by continuous culture, which comprises the steps of:
- (a) cultivating a microorganism capable of producing pullulan at a pH exceeding 2.0 but not higher than 4.0 in a nutrient culture medium containing 10-20 w/v % of a saccharide to produce and accumulate pullulan in said nutrient culture medium while controlling the viscosity of said nutrient culture medium to a level below 30 centipoise;
- and
- (b) purifying and recovering a product which contains pullulan.
Dependencies are inferred from the claim text.