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US 5,516,911 AGranted
Fluorescent intracellular calcium indicators
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsRobert E. London, Louis A. Levy, Elizabeth Murphy
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Abstract
A new class of calcium-specific, NMR-active and/or fluorescent indicators having a high dissociation constant is described. Methods of determining intracellular calcium ion concentration using .sup.19 F NMR spectroscopy or optical methods are also provided.
Claims5
A compound of the formula:
- ##STR24## wherein:
- R is selected from the group consisting of hydrogen, acetoxymethyl, and a sodium, potassium, ammonium or lithium cation;
- R' and R" are selected independently from the group consisting of hydrogen and lower alkyl, or R' and R", and the carbon atoms to which they are bonded, may be constituents of a 5-membered or 6-membered carbocyclic or heterocyclic ring;
- R.sub.1, R.sub.2, R.sub.7 and R.sub.8 are selected independently from the group consisting of hydrogen, lower alkyl, aryl, substituted alkyl, substituted aryl, alkoxy, amino, carboxyl, cyano, halogen, hydroxyl, nitro, and chromophoric or fluorescent substituents that are capable of functioning as optical indicators;
- and
- R.sub.3 -R.sub.6 are fluorine.
3 more elements
A compound having the formula:
- ##STR26## wherein:
- R is selected from the group consisting of hydrogen, acetoxymethyl, and a sodium, potassium, ammonium or lithium cation;
- R' and R" are selected independently from the group consisting of hydrogen and lower alkyl, or R' and R", and the carbon atoms to which they are bonded, may be constituents of a 5-membered or 6-membered carbocyclic or heterocyclic ring;
- and
- R.sub.1 -R.sub.8 are selected independently from the group consisting of hydrogen, lower alkyl, aryl, substituted alkyl, substituted aryl, alkoxy, amino, carboxyl, cyano, halogen hydroxyl, nitro, and chromophoric or fluorescent substituents that are capable of functioninq as optical indicators;
- provided at least one of R.sub.1 -R.sub.8 is selected independently from the group consisting of chromophoric or fluorescent substituents that are capable of functioning as optical indicators and at least one of R.sub.4 and R.sub.5 is fluorine.
3 more elements
Dependencies are inferred from the claim text.