Process for preparing cephalosporin compounds
AssigneeBecton, Dickinson and Company
InventorsJ. Michael Quante, Randal A. Hoke, Patrick D. Mize, Daniel L. Woodard, O. Elmo Millner, deceased
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Abstract
Chromogenic and fluorogenic substrates for .beta.-lactamase, methods for synthesis thereof and methods for detecting .beta.-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent .beta.-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by .beta.-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.
Claims3
A method of making cephalothin aminomethyl coumarin carbamate or cefotaxime aminomethyl coumarin carbamate comprising:
- a) reacting desacetyl cefotaxime or desacetyl cephalothin with 4-methyl-7-isocyano coumarin in the presence of an organic solvent;
- b) precipitating the cephalothin aminomethyl coumarin carbamate or the cefotaxime aminomethyl coumarin carbamate by addition of a mixture of acetone and ethyl acetate, and;
- c) isolating the precipitated cephalothin aminomethyl coumarin carbamate or cefotaxime aminomethyl coumarin carbamate.
Dependencies are inferred from the claim text.