Method for the selective dissolution of cancer cells using a composition derived from the photosynthetic system of plants and mammalian embryonic tissue

A pharmaceutical composition, comprising:

• a) a protein fraction with oxygen activity and derived from the photosynthetic system of plants, said protein fraction having a molecular weight of approximately 67000 d.+-.10000 d as determined by SDS gradient gel electrophoresis in the presence of a marker for the pH range of 4.7 to 10.6 being formed by a process comprising the steps of:
• isolating chloroplasts from autotrophic plants or autotrophic lower organisms;
• washing the isolated chloroplasts;
• treating the isolated and washed chloroplasts with a hypotonic ethanol solution to effect their lysis;
• removal of formed sediment;
• resolution of the alcoholic solution by support-free deflection electrophoresis to obtain a yellowish, oxygen-liberating protein fraction, and optionally subsequent concentration of this fraction by ultrafiltration, dialyale of the ultrafiltration retentate against water and freeze-drying;
• and
• b) a protein mixture derived from calf or sheep embryos and fetuses, said protein mixture comprising proteins yielding a main band at 62000 d.+-.10000 d and secondary bands at 43000 d+10000 d and and and 13800 d.+-.5000 d in SDS polyacrylamide gel electrophoresis, and being formed by a process comprising the steps of
• comminuting deep-frozen calf or sheep embryos or fetuses under sterile conditions in the presence of a polar water-containing organic solvent;
• and
• removing components which are capable of separation from the organic solvent by sedimentation or sterile filtration;
• fine filtration and microfiltration of the organic solution which has been freed from the components which are capable of separation;
• filling the filtrate into containers without further resolution into protein fractions, and optionally freeze-drying, with all the process steps mentioned being carried out under sterile conditions,
• as a combined preparation for simultaneous or sequential administration by injection or infusion in the treatment of cancer cells in live organisms of warm-blooded species.

A process for selective dissolution of cancer cells in live organisms of warm-blooded species, which process comprises administering by injection or infusion to a patient simultaneously or sequentially as a combined pharmaceutical preparation an effective amount of

• a) an amount of a protein fraction with oxygen activity and derived from the photosynthetic system of plants, said protein fraction having a molecular weight of approximately 67000 d.perp.10000 d as determined by SDS gradient gel electrophoresis in the presence of a marker for the pH range of 4.7 to 10.6 and being formed by a process comprising the steps of:
• isolating chloroplasts from autotrophic plants or autotrophic lower organisms;
• washing the isolated chloroplasts;
• treating the isolated and washed chloroplasts with a hypotonic ethanol solution to effect their lysis;
• removal of formed sediment;
• resolution of the alcoholic solution by support-free deflection electrophoresis to obtain a yellowish, oxygen-liberating protein fraction, and optionally subsequent concentration to this fraction by ultrafiltration, dialysis of the ultrafiltration retentate against water and freeze-drying;
• and
• b) an amount of a protein mixture derived from calf or sheep embryos and fetuses, said protein mixture comprising proteins yielding a main band at 62000 d.+-.10000 d and secondary bands at 43000 d.perp.10000 d and and 13800 d.+-.5000 d in SDS polyacrylamide gel electrophoresis, and being formed by a process comprising the steps of
• comminuting deep-frozen calf or sheep embryos or fetuses under sterile conditions in the presence of a polar water-containing organic solvent;
• and
• removing components which are capable of separation from the organic solvent by sedimentation or sterile filtration;
• fine filtration and microfiltration of the organic solution which has been freed from the components which are capable of separation;
• filling the filtrate into containers without further resolution into protein fractions, and optionally freeze-drying, with all the process steps mentioned being carried out under sterile conditions.