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US 5,516,703 AGranted
Coating of hydrophobic surfaces to render them protein resistant while permitting covalent attachment of specific ligands
AssigneeUniversity of Utah
InventorsKarin M. E. Caldwell, Per J. E. Carlsson, Jenq-Thun Li
Life of this patent
Estimating the term…
Abstract
A surface of specific reactivity is formed by adsorbing a modified block polymeric surfactant of the Pluronic-type, i.e., containing pendant poly(ethylene oxide) blocks attached at an end to poly(propylene oxide) center blocks and with specifically reactive groups at the unattached ends of the poly(ethylene oxide) blocks, upon a hydrophobic surface.
Claims29
A method for forming a hydrophilic surface having a specific chemical reactivity on a hydrophobic substrate, which method comprises:
- (a) providing a hydrophobic substrate, the hydrophobic substrate characterized as having a surface water contact angle greater than about 60.degree.,
- (b) providing a polymeric surfactant with substantially no reactivity to biopolymers, the surfactant characterized as having at least one pendant hydrophilic block (PEO) with one end attached directly or by bridging structures to a hydrophobic block (PPO) and the other end attached to pendant --O--H groups,
- the number of the pendant hydrophilic blocks (PEO) with attached --O--H groups (H--O--PEO) and the hydrophobic blocks (PPO) in the molecule being represented by:
- e(H--O--PEO):b(PPO)
- where b is an integer between 1 and 3, e is an integer between 1 and 6, and where PEO is the hydrophilic block and is of the formula:
- --(--C.sub.2 H.sub.4 --O--).sub.u --
- where u is the same or different in each PEO block in the molecule and is greater than 50, where PPO is the hydrophobic block and is of the formula:
- --(--C.sub.3 H.sub.6 --O--).sub.v --
- where v is the same or different in each PEO block in the molecule and is greater than 25,
- (c) reacting at least a portion of the --OH groups in the HO--PEO groups of the polymeric surfactant to provide reactive groups (R) on the pendant ends of at least a portion of the PEO groups to form a modified polymeric surfactant having pendant hydrophilic blocks (PEO) with one end attached to the hydrophobic block (PPO) and the other end attached to pendant reactive --O--R groups, R being stable in water and having the specific chemical reactivity that is provided to the surface,
- the number in the modified polymeric surfactant of the pendant hydrophilic blocks with attached --O--R groups (R--O--PEO--), and the pendant hydrophilic blocks with attached unreacted --O--H groups (HO--PEO--), and the hydrophobic block (PPO) in the molecule being represented by:
- c (HO--PEO):
- d (R--O--PEO):
- b (PPO)
- where b is an integer from 1 to 3, (c+d) is e, c is an integer between 0 and 5, and d is at least 1, and
- (d) adsorbing the PPO portions of the modified surfactant of step (c) upon the hydrophobic surface such that said PPO portions arc adsorbed upon the surface, and HO--PEO and R--O--PEO groups of the molecule extend from the surface.
13 more elements
A method for forming on a hydrophobic substrate a hydrophilic surface having a predetermined density of specific chemical reactivity towards a thiolated protein, which method comprises:
- (a) providing a hydrophobic substrate, the hydrophobic substrate characterized as having a surface water contact angle greater than about 60.degree.,
- (b) providing a polymeric surfactant with substantially no reactivity to biopolymers, the surfactant characterized as having at least one pendant hydrophilic block (PEO) with one end attached directly or by bridging structures to a hydrophobic block (PPO) and the other end attached to pendant--O--H groups,
- the number of the pendant hydrophilic blocks (PEO) with attached --O--H groups (H--O--PEO) and the hydrophobic blocks (PPO) in the molecule being represented by:
- e (H--O--PEO):
- b (PPO)
- where b is an integer between 1 and 3, e is an integer between 1 and 6, and where PEO is the hydrophilic block and is of the formula:
- --(--C.sub.2 H.sub.4 --O--).sub.u --
- where u is the same or different in each PEO block in the molecule and is greater than 50, where PPO is the hydrophobic block and is of the formula:
- --(--C.sub.3 H.sub.6 --O--).sub.v --
- where v is the same or different in each PEO block in the molecule and is greater than 25,
- (c) reacting at least a portion of the --OH groups in the HO--PEO groups of the polymeric surfactant to provide reactive groups (R) on the pendant ends of at least a portion the PEO groups to form a modified polymeric surfactant having pendant hydrophilic blocks (PEO) with one end attached to the hydrophobic block (PPO) and the other end attached to pendant reactive --O--R groups, R containing a thiopyridyl group and being chosen from the group consisting of:
- ##STR14## wherein n is greater than 1, preferably between 1 and 4, and R.sub.1 is --NO.sub.2 or --H, the number in the modified polymeric surfactant of the pendant hydrophilic block with attached --O--R groups (R--O--PEO--), and the pendant hydrophilic block with attached unreacted --O--H groups (HO--PEO--), and the hydrophobic block (PPO) in the molecule being represented by;
- c (HO--PEO):
- d (R--O--PEO):
- b (PPO)
- where b is an integer from 1 to 3, (c+d) is e, c is an integer between 0 and 5, and d is at least 1, and
- (d) adsorbing the PPO portions of the modified surfactant of step (c) upon the hydrophobic surface such that said PPO portions are adsorbed upon the surface, and HO--PEO and R--O--PEO groups of the molecule extend from the surface.
14 more elements
Dependencies are inferred from the claim text.