Endo-xyloglucan transferase
AssigneeTakara Shuzo Co., Ltd.
InventorsKazuhiko Nishitani, Kazuhide Okazawa, Kiyozo Asada, Ikunoshin Kato
Life of this patent
Estimating the term…
Abstract
Endo-xyloglucan transferases responsible for growth of plant cell wall, genes coding for the enzymes, a method of transferring xyloglucan molecules by using the enzyme, and methods of using the gene are described.
Claims16
An isolated, purified DNA fragment coding for an endo-xyloglucan transferase, which comprises a DNA having the sequence shown in SEQ ID NO:
- 15.
An isolated, purified DNA fragment, which codes for a protein having endo-xyloglucan transferase activity, and which comprises a DNA sequence whose complementary DNA sequence hybridizes with the DNA sequence shown in SEQ ID NO:
- 15.
An isolated, purified DNA fragment, which comprises a DNA having the sequence shown in SEQ ID NO:
- 16, 17, 18, or 19.
A method of using a DNA fragment comprising a DNA having the sequence shown in SEQ ID NO. 1 or 15 as a probe to isolate a recombinant DNA clone encoding an endo-xyloglucan transferase, comprising the steps of:
- (a) isolating genomic DNA or cDNA from a plant;
- (b) constructing a genomic DNA or cDNA library consisting of a plurality of recombinant DNA clones each containing a different segment of the genomic DNA or cDNA obtained in step (a);
- (c) contacting the genomic DNA or cDNA library of recombinant DNA clones obtained in step (b) with a DNA probe comprising a DNA having the sequence shown in SEQ ID NO. 1 or 15 respectively to hybridize the DNA probe to complementary sequences in the genomic DNA or cDNA library of clones;
- (d) detecting a clone to which the DNA probe has hybridized;
- and
- (e) isolating the clone detected in step (d).
An isolated, purified antisense DNA fragment, which comprises a DNA having a sequence which is complementary to SEQ ID NO:
- 15.
An isolated, purified antisense DNA fragment, which comprises a DNA having the sequence shown in SEQ ID NO:
- 1, 2, 3, 4, or 5.
An isolated, purified antisense RNA, which comprises an RNA having a sequence complementary to an RNA transcribed from SEQ ID NO:
- 15.
An isolated, purified antisense RNA, which comprises an RNA having the sequence shown in SEQ ID NO:
- 6, 7, 8, 9, or 10.
Dependencies are inferred from the claim text.