Penicillin V amidohydrolase gene from Fusarium oxysporum
AssigneeBristol-Myers Squibb Company
InventorsShu-Jen Chiang, William V. Burnett, Jr., Sean M. Tonzi
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Abstract
Nucleic acids coding for penicillin V amidohydrolase (PVA) from Fusarium oxysporum. Also, expression vectors, host cells and a method for production of PVA.
Claims71
An isolated nucleic acid molecule having a sequence coding for the amino acid sequence of SEQ. ID. NOS.:
- 19 or 22.
An isolated nucleic acid molecule having a sequence complementary to a nucleic acid sequence coding for the amino acid sequence of SEQ. ID. NOS.:19 or 22.
An isolated nucleic acid molecule having a sequence capable of hybridizing under stringent conditions to a nucleic acid having a sequence complementary to a nucleic acid sequence coding for the amino acid sequence of SEQ. ID. NOS.:19 or 22.
An isolated DNA molecule having a sequence coding for the amino acid sequence of SEQ. ID. NO.:19.
An isolated DNA molecule having a sequence capable of hybridizing under stringent conditions to a nucleic acid having a sequence complementary to a nucleic acid sequence coding for the amino acid sequence of SEQ. ID. NO.:19.
An isolated DNA molecule having a sequence coding for the amino acid sequence of SEQ. ID. NO.:22.
An isolated DNA molecule having a sequence capable of hybridizing under stringent conditions to a nucleic acid having a sequence complementary to a nucleic acid sequence coding for the amino acid sequence of SEQ. ID. NO.:22.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NO.:18.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NO.:21.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NO.:
- 11.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NO.:
- 23.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NOS.:
- 14, 15, 16, or 17.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NOS.:9 or 10.
An isolated DNA molecule having the nucleotide sequence of SEQ. ID. NOS.:26 or 30.
An isolated polypeptide having the amino acid sequence of SEQ. ID. NOS.:1, 2, 3, 4, 5, 6, 8, or 12.
An expression vector comprising a nucleic acid sequence coding for the amino acid sequence of SEQ. ID. NOS.:19 or 22.
An expression vector comprising a promoter having the DNA sequence of SEQ. ID. NO.:23.
An expression vector having the designation pBMFXPVA6.
An expression vector having the designation pBMFXPVA7.
An expression vector having the designation pBMPVA-P.
An expression vector having the designation pBMPVA-M.
A biologically pure culture of Escherichia coil ATCC 69720.
A biologically pure culture of Escherichia coil ATCC 69721.
A biologically pure culture of Escherichia coil ATCC 69772.
Dependencies are inferred from the claim text.