Method for producing the SSPI restriction endonuclease and methylase

Abstract

The present invention is directed to a method for cloning and producing the SspI restriction endonuclease by 1) introducing the restriction endonuclease gene from Sphaerotilus species into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the SspI restriction endonuclease, and 3) purifying the SspI restriction endonuclease from the fermented host which contains the vector encoding and expressing the SspI restriction endonuclease activity.

Dependencies are inferred from the claim text.