Stabilized peroxidase compositions and antibody compositions
AssigneeKonica Corporation
InventorsMasahiko Yamasaki, Morito Uemura, Shinya Yoshida
Life of this patent
Estimating the term…
Abstract
Disclosed are a peroxidase composition and an antibody composition with high stability. In the compositions of the present invention, a substituted benzene ring having at least one hydroxyl group and at least one substituting group selected from the group consisting of groups with a Hammett sigma value .sigma. p of not more than -0.20, groups with a Hammett sigma value .sigma. p of not less than +0.24 and amide group is contained as a stabilizer for stabilizing the activity of peroxidase or the antibody.
Claims13
A peroxidase composition consisting essentially of:
- (a) a peroxidase or a peroxidase conjugated with an immunologically active substance;
- (b) a buffer;
- (c) a benzene derivative as a stabilizer;
- and
- (d) one of serum of animal, serum component, carbohydrate and water-soluble macromolecule,
- wherein said benzene derivative is selected from the group consisting of:
- hydroquinone monomethyl ether, hydroquinone monoethyl ether, hydroquinone monophenyl ether, o-methoxyphenol, o-ethoxyphenol, 2,6-dimethoxyphenol, hydroquinone, N-methyl-p-hydroxyaniline, N,N-dimethyl-p-hydroxyaniline, N-(p-hydroxyphenyl)glycine, p-hydroxyacetophenone, 2,4-dihydroxyacetophenone, p-hydroxybenzophenone, p-hydroxybenzoic acid, m-hydroxybenzoic acid, o-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,6-dihydroxy benzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 2,3,4-trihydroxybenzoic acid, 2,3,5-trihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 4-hydroxy-3,5-dimethoxybenzoic acid, 4-hydroxyphthalic acid, 2-hydroxyterephthalic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, n-propyl p-hydroxybenzoate, ethyl 3,4-dihydroxy benzoate, p-hydroxybenzamide, p-hydroxybenzoylethylamine, 1-naphthol-2-carboxylic acid, 3-naphthol-2-carboxylic acid, p-hydroxyacetoanilide, p-hydroxybenzenesulfinyl methane, p-hydroxybenzene sulfonamide, p-sulfonylphenol and p-nitrophenol.
An antibody composition consisting essentially of:
- (a) an antibody;
- (b) a buffer;
- (c) a benzene derivative as a stabilizer;
- and
- (d) one of serum of animal, serum component, carbohydrate and water-soluble macromolecule,
- wherein said benzene derivative is selected from the group consisting of:
- hydroquinone monomethyl ether, hydroquinone monoethyl ether, hydroquinone monophenyl ether, o-methoxyphenol, o-ethoxyphenol, 2,6-dimethoxyphenol, hydroquinone, N-methyl-p-hydroxyaniline, N,N-dimethyl-p-hydroxyaniline, N-(p-hydroxyphenyl)glycine, p-hydroxyacetophenone, 2,4-dihydroxyacetophenone, p-hydroxybenzophenone, p-hydroxybenzoic acid, m-hydroxybenzoic acid, o-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,6-dihydroxy benzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 2,3,4-trihydroxybenzoic acid, 2,3,5-trihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 4-hydroxy-3,5-dimethoxybenzoic acid, 4-hydroxyphthalic acid, 2-hydroxyterephthalic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, n-propyl p-hydroxybenzoate, ethyl 3,4-dihydroxy benzoate, p-hydroxybenzamide, p-hydroxybenzoylethylamine, 1-naphthol-2-carboxylic acid, 3-naphthol-2-carboxylic acid, p-hydroxyacetoanilide, p-hydroxybenzenesulfinyl methane, p-hydroxybenzene sulfonamide, p-sulfonylphenol and p-nitrophenol.
Dependencies are inferred from the claim text.