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US 5,516,671 AGranted
Method of controlling plant pathogens
AssigneeMonsanto Company
InventorsEllen B. Lawrence, Elaine B. Levine, Dilip M. Shah
Life of this patent
Estimating the term…
Abstract
Plants can be transformed to express glucose oxidase from Aspergillus sp. and be made resistant to bacterial and fungal pathogens. Optionally, the plants may also express invertase to increase the available glucose as a substrate for enzymatic activity.
Claims13
A recombinant, double-stranded DNA molecule comprising in operative sequence:
- a) a promoter which functions in plant cells to cause the production of an RNA sequence;
- b) a structural coding sequence that encodes for production of Aspergillus glucose oxidase;
- and
- c) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence.
1 more element
A method of producing genetically transformed, disease resistant plants, comprising the steps of:
- a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising
- (i) a promoter which functions in plant cells to cause the production of an RNA sequence;
- (ii) a structural coding sequence that causes the production of Aspergillus glucose oxidase;
- (iii) a 3' non-translated region which functions in said plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence;
- b) obtaining transformed plant cells;
- and
- c) regenerating from the transformed plant cells genetically transformed plants which express Aspergillus glucose oxidase in an amount effective to reduce damage due to infection by a bacterial or fungal pathogen.
4 more elements
A genetically transformed, disease resistant plant comprising a recombinant, double-stranded DNA molecule comprising in operative sequence:
- a) a promoter which functions in plant cells to cause the production of an RNA sequence;
- b) a structural coding sequence that encodes for production of Aspergillus glucose oxidase;
- and
- c) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence.
1 more element
Dependencies are inferred from the claim text.