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US 5,516,669 AGranted
Transposon consisting of a fragment of the R plasmid pCxM82B, test vectors containing this transposon, and methods of mutagenesis
AssigneeDegussa AG
InventorsFriedrich Kassing, Andreas Schafer, Jorn Kalinowski, Alfred Puhler
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Abstract
The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this transposon, and to methods for the mutagenesis of gram-positive bacteria by means of the transfer of these vectors and the generation of auxotrophic mutants.
Claims13
A transposon consisting of a DNA segment of the R plasmid pCxM82B wherein said DNA segment comprises:
- (a) a segment of DNA necessary for integration;
- (b) a segment of DNA coding for tetracycline resistance, and
- (c) a segment of DNA coding for erythromycin resistance,
- wherein said DNA segment of the R plasmid pCxM82B comprises an approximately 14.4 kb DNA segment delimited by a SalI cleavage site (coordinate 30.4 kb) and a HindIII cleavage site (coordinate 44.8 kb) of pCxM28B set forth in FIG. 1.
1 more element
Dependencies are inferred from the claim text.