Method for decreasing seed storage proteins and for transforming plants
AssigneesJapan Tobacco Inc., Rice Breeding Research Laboratories
InventorYoshiyuki Maruta
Life of this patent
Estimating the term…
Abstract
A method for decreasing a seed storage protein in seeds by a genetic engineering method and a method for transforming plants are disclosed. In the method of the present invention, a gene which is a template of a mRNA having a nucleotide sequence complementary to the mRNA of the seed storage protein is introduced in a plant; and the gene in seeds of the plant is transcribed to inhibit translation of the mRNA of the seed storage protein, thereby decreasing the seed storage protein in the seeds. In the method for transforming plants according to the present invention, a plant is transformed with a vector containing a glutelin promoter and a foreign gene.
Claims13
A method for decreasing the amount of glutelin in plant seeds, comprising:
- introducing into a rice plant a gene which is a template for the transcription of an antisense RNA against glutelin;
- and
- transcribing said gene in seeds from said rice plant to inhibit translation of mRNA of glutelin, thereby decreasing the amount of glutelin in said seeds in comparison to the amount of glutelin contained in seeds from said rice plant into which said gene has not been introduced.
A method for decreasing the amount of glutelin in rice seeds, comprising:
- introducing into a rice plant a glutelin gene which acts as a template for the transcription of an antisense RNA against glutelin by transforming said rice plant with plasmid pANG2 or plasmid pANG3, and
- transcribing said glutelin gene in seeds of said rice plant utilizing a glutelin gene promoter to inhibit translation of mRNA of said glutelin, thereby decreasing the amount of said glutelin in said seeds in comparison to the amount of said glutelin contained in seeds of said rice plant into which said glutelin gene has not been introduced.
Dependencies are inferred from the claim text.