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US 5,516,667 AGranted
Chilling-resistant plants and their production
AssigneeKirin Beer Kabushiki Kaisha
InventorOsamu Nishizawa
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AssigneeKirin Beer Kabushiki Kaisha
InventorOsamu Nishizawa
Estimating the term…
The present invention relates to the genetic engineering of higher plants to confer chilling resistance. Provided is a higher plant which contains more unsaturated fatty acids in membrane lipids than are inherent to that plant species, and a process for producing the same. A preferred embodiment of such plant is a transgenic plant expressing a polypeptide with a glycerol 3-phosphate acyltransferase activity that has a higher substrate selectivity for oleoyl-(acyl-carrier-protein) (oleoyl-ACP) than for palmitoyl-(acyl-carrier-protein) (palmitoyl-ACP). In another aspect, there are provided higher plants with a lowered critical temperature for chilling injury, and a process to produce the same. A preferred embodiment of such plant is a transgenic plant whose phosphatidylglycerol contains reduced amount of saturated molecular species due to the expression of a polypeptide with a glycerol 3-phosphate acyltransferase activity that has a higher substrate selectivity for oleoyl-ACP than for palmitoyl-ACP.
A transgenic higher plant characterized by the presence in at least one of its lipid classes of a higher proportion of unsaturated fatty acids than inherently present in species of said plant and by the presence in its cells of an exogenous DNA sequence encoding a polypeptide with a glycerol 3-phosphate acyltransferase activity having a higher substrate selectivity for oleoyl-(acyl-carrier-protein) than for palmitoyl-(acyl-carrier-protein).
A process for increasing the unsaturated fatty acid content in lipids of a higher plant species comprising introducing into the cells thereof an exogenous DNA sequence encoding a polypeptide with a glycerol 3-phosphate acyltransferase activity having a higher substrate selectivity for oleoyl-(acyl-carrier-protein) than for palmitoyl-(acyl-carrier-protein).
A transgenic higher plant characterized by having a lowered critical temperature for chilling injury than that inherent in species of said plant and by containing in the biomembranes of its cells a decreased proportion of saturated phosphatidylglycerol molecular species and by the presence in its cells of an exogenous DNA sequence encoding a polypeptide with a glycerol 3-phosphate acyltransferase activity having a higher substrate selectivity for oleoyl-(acyl-carrier-protein) than for palmitoyl-(acyl-carrier-protein).
A process for lowering the critical temperature for chilling injury of a higher plant species comprising decreasing the content of saturated phosphatidylglycerol molecular species in the biomembranes of its cells and introducing into its cells an exogenous DNA sequence encoding a polypeptide with a glycerol 3-phosphate acyltransferase activity having a higher substrate selectivity for oleoyl-(acyl-carrier-protein) than for palmitoyl-(acyl-carrier-protein).
Dependencies are inferred from the claim text.