Microorganisms, their use and method of producing L-.alpha.-amino acids
AssigneeDegussa AG
InventorsFritz Wagner, Dirk Volkel, Andreas Bommarius, Karlheinz Drauz
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Abstract
The invention relates to novel microorganisms, their use and method of producing L-.alpha.-amino acids. In particular, microorganisms DSM 7329 and 7330 are suitable for the production of L-.alpha.-amino acids from corresponding hydantoins or carbamoyl-.alpha.-amino acids. These novel microorganisms are simple to cultivate and make possible high L-.alpha.-amino acid yields from different substrates.
Claims10
Isolated Arthrobacter microorganism DSM 7329.
Isolated Arthrobacter microorganism DSM 7330.
A method for producing L-.alpha.-amino acids, comprising the step of enzymatically converting one or more of the compounds selected from the group consisting of D-5-monosubstituted hydantoin, L-5-monosubstituted hydantoin, D,L-5-monosubstituted hydantoin, D-N-carbamoyl-.alpha.-amino acid, L-N-carbamoyl-.alpha.-amino acid, and D, L-N-carbamoyl-.alpha.-amino acid into the corresponding L-.alpha.-amino acid in the presence of one or both of Arthobacter microorganism DSM 7329 and Arthobacter microorganism DSM 7330.
A method for producing L-.alpha.-amino acids, comprising the step of enzymatically converting one or more of the compounds selected from the group consisting of D-5-monosubstituted hydantoin, L-5-monosubstituted hydantoin, D,L-5-monosubstituted hydantoin, D-N-carbamoyl-.alpha.-amino acid, L-N-carbamoyl-.alpha.-amino acid, and D, L-N-carbamoyl-.alpha.-amino acid into the corresponding L-.alpha.-amino acid, in the presence of enzymes produced from one or both of Arthrobacter microorganism DSM 7329 and Arthrobacter microorganism DSM 7330.
An isolated Arthrobacter microorganism 7329 which contains a gene coding for a N-carbamoyl-L-amino acid amidohydrolase hydantoinase or hydantoin racemase.
An isolated Arthrobacter microorganism 7330 which contains a gene coding for a N-carbamoyl-L-amino acid amino hydrolase hydantoinase or hydantoin racemase.
Dependencies are inferred from the claim text.