DNA encoding cytokines that bind the cell surface receptor hek
AssigneeImmunex Corporation
InventorsM. Patricia Beckmann, Douglas P. Cerretti
Life of this patent
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Abstract
Hek ligand (hek-L) polypeptides as well as DNA sequences, vectors and transformed host cells useful in providing hek-L polypeptides. The hek-L polypeptides bind to a cell surface receptor (hek) that is a member of the receptor tyrosine kinase family. Hek is expressed on cells that include certain tumor cell lines. The hek-L polypeptides also bind a distinct receptor tyrosine kinase known elk.
Claims27
An isolated DNA encoding a hgk ligand/hek-L) protein capable of binding hek, wherein said DNA comprises a nucleotide sequence selected from the group consisting of nucleotides 83-796, 83-745, 140-796, and 140-745 of SEQ ID NO:
- 1.
An isolated DNA encoding a hek-L protein capable of binding hek, wherein said DNA comprises a nucleotide sequence selected from the group consisting of nucleotides 28-630, 28-573, 94-630, and 94-573 of SEQ ID NO:3.
An isolated DNA encoding a bek-L protein capable of binding hek, wherein said hek-L comprises an amino acid sequence selected from the group consisting of amino acids 1-202 and 1-219 of SEQ ID NO:2 and amino acids 1-160 and 1-179 of SEQ ID NO:4.
An isolated DNA encoding a fusion protein comprising a hek-L capable of binding hek and an Fc polypeptide, wherein said hek-L comprises an amino acid sequence selected from the group consisting of amino acids 1-202 of SEQ ID NO:2 and amino acids 1-160 of SEQ ID NO:4.
An isolated DNA encoding a hek-L polypeptide, wherein said hek-L polypeptide comprises the amino acid sequence of residues 1-219 of SEQ ID NO:2.
An isolated DNA encoding a hek-L polypeptide, wherein said hek-L polypeptide comprises the amino acid sequence of residues 1-179 of SEQ ID NO:4.
An isolated DNA encoding a hek-L polypeptide, wherein said hek-L polypeptide comprises an amino acid sequence selected from the group consisting of:
- a) amino acids z through x of SEQ ID NO:2, wherein z is an amino acid in positions 1 to 12 of SEQ ID NO:2 and x is an amino acid in positions 193 to 219 of SEQ ID NO:2;
- and
- b) amino acids z' through y of SEQ ID NO:4, wherein z' is an amino acid in positions 1 to 4 of SEQ ID NO:4 and y is an amino acid in positions 147 to 179 of SEQ ID NO:4.
An isolated DNA selected from the group consisting of:
- a) a DNA encoding a fragment of a hek-L polypeptide comprising residues 1-219 of SEQ ID NO:2, and
- b) a DNA encoding a fragment of a hek-L polypeptide comprising residues 1-179 of SEQ ID NO:4,
- wherein said fragment is capable of binding hek.
An isolated DNA encoding a fusion protein comprising a hek-L and an Fc polypeptide, wherein said hek-L comprises an amino acid sequence selected from the group consisting of:
- a) a fragment of a hek-L polypeptide comprising residues 1-219 of SEQ ID NO:2, and
- b) a fragment of a hck-L polypeptide comprising residues 1-179 of SEQ ID NO:4, wherein said fragment is a soluble hek-I., capable of binding hek.
Dependencies are inferred from the claim text.