Baculovirus vectors for expression of secretory and membrane-bound proteins
AssigneeCambridge Biotech Corporation
InventorsCheryl I. Murphy, Elihu Young
Life of this patent
Estimating the term…
Abstract
The invention provides Baculovirus vectors comprised of a promoter upstream from a signal peptide. The Baculovirus vectors allow for the expression of a glycosylated protein in the late term of Baculovirus infection. Methods for the construction of such a Baculovirus vector are also provided. In addition, methods for producing large amounts of a desired glycosylated protein in the late term infection of Baculovirus infection are also provided.
Claims18
A vector for expression and secretion of foreign proteins in insect host cells comprising a Baculovirus promoter operably linked to an insect virus signal peptide coding region from a baculovirus protein, wherein said signal peptide is derived from a 60 kD protein encoded by the AcMNPV egt gene of Baculovirus.
A vector for expression and secretion of foreign proteins in insect host cells comprising a Baculovirus promoter operably linked to an insect virus signal peptide coding region from a baculovirus protein, wherein said signal peptide is derived from the Baculovirus glycoprotein p67.
A virus comprising the DNA sequence encoding a fusion protein, said fusion protein further comprising a signal peptide derived from a 60 kD protein encoded by the AcMNPV egt gene of Baculovirus or a signal peptide derived from the Baculovirus p67 gene.
Dependencies are inferred from the claim text.