Production of a new hirudin analog and anticoagulant pharmaceutical composition containing the same
AssigneeNippon Mining Co., Ltd.
InventorsSatoru Misawa, Hitoshi Matsuda, Yoshifumi Inoue, Hideyuki Furuya
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Abstract
Provided is a chimeric HV1/HV3 hirudin analog useful as an anti-coagulant, exhibiting higher anti-thrombin activity and less tendency to cause bleeding than hirudin HV1. Also provided are a method of producing hirudins and hirudin analogs, as well as other proteins, employing secretion vectors, and transformed microorganisms such as E. coli. Pharmaceutical compositions comprising various hirudins or the chimeric HV1/HV3 hirudin analog of the present invention and a pharmaceutically acceptable carrier are also disclosed.
Claims10
A hirudin analog, HV1C3, having the amino acid sequence of SEQ ID NO:1.
A secretion vector, comprising a DNA sequence comprising a replication origin of a pUC plasmid, a tac or a trp promoter, a DNA sequence encoding a signal peptide, and a DNA sequence encoding hirudin analog HV1C3 having the amino acid sequence of SEQ ID NO:1 linked directly downstream from said DNA sequence encoding said signal peptide, all linked in an operable manner.
Dependencies are inferred from the claim text.