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US 5,516,649 AGranted
Process for the production of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione from ergosterol with mycobacterium
AssigneeSchering Corporation
InventorsAlfred Weber, Mario Kennecke
Life of this patent
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Abstract
A process for the production of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione of the general formula ##STR1## wherein . . . . . symbolizes a single bond or a double bond, characterized in that ergosterol ##STR2## is fermented with a culture of a microorganism strain capable of side-chain degradation of sterols.
Claims6
A process for the production of 4-androstene-3,17-dione of the formula ##STR3## comprising:
- culturing Mycobacterium spec. NRRL B-3805 in the absence of a 9.alpha.-hydroxylation inhibitor;
- adding ergosterol to said cultured Mycobacterium, wherein said ergosterol is dissolved in a solvent selected from the group consisting of methanol, ethanol, glycol monomethyl ether, dimethylformamide, and dimethylsulfoxide;
- further culturing said strain in the absence of said inhibitor;
- and
- isolating the resultant 4-androstene-3,17-dione.
2 more elements
A process for the production of 1,4-androstadiene-3,17-dione of the formula ##STR4## comprising:
- culturing Mycobacterium spec. NRRL B-3683 in the absence of a 9.alpha.-hydroxylation inhibitor;
- adding ergosterol to said cultured Mycobacterium, wherein said ergosterol is dissolved in a solvent selected from the group consisting of methanol, ethanol, glycol, monomethyl ether, dimethylformamide, and dimethylsulfoxide;
- further culturing said strain in the absence of said inhibitor;
- and
- isolating the resultant 1,4-androstadiene-3,17-dione.
2 more elements
Dependencies are inferred from the claim text.