Immunoassay analytical elements containing vanadium IV(V.sup.+4)ions
AssigneeJohnson & Johnson Clinical Diagnostics, Inc.
InventorsDaniel S. Daniel, David A. Hilborn, Calvin R. Messing
Life of this patent
Estimating the term…
Abstract
A dry immunoassay analytical element, for assaying a ligand, comprising a support bearing: (a) a labeled ligand zone; (b) a spreading zone; and (c) a receptor zone containing a fixed concentration of an immobilized receptor for the ligand and the labeled ligand and the receptor is covalently bonded to polymeric beads having a diameter in the range of 0.1 to 5 .mu.m; characterized in that the element contains a compound containing a vanadium IV (V.sup.+4) ion and the zones can be in the same or separate layers.
Claims25
In a dry immunoassay analytical element, for assaying a ligand, comprising a support bearing:
- a) a labeled ligand zone containing an enzyme-labeled ligand;
- b) a spreading zone;
- and
- c) a receptor zone containing a fixed concentration of an immobilized receptor for the ligand and the labeled ligand wherein the receptor is covalently bonded to polymeric beads having a diameter in the range of 0.1 to 5 um;
- the improvement wherein the spreading zone comprises a compound containing a vanadium IV (V.sup.+4) ion, said compound being a) compatible with said receptor and said labeled ligand to permit said immunoassay to take place and b) selected from the group consisting of vanadyl and vanadite compounds,
- said compound being further present in said element within a range of from 0.025 to 0.5 mmoles per m.sup.2,
- said zones being in one or more separate layers.
Dependencies are inferred from the claim text.