US 5,516,641 A · Method for detection of specific nucleic acid sequences

Abstract

A kit is disclosed for a method for detecting the presence of a target polynucleotide sequence. The kit comprises a first polynucleotide sequence and a second polynucleotide sequence complementary to non-contiguous portions of a target polynucleotide sequence, which first and second sequences are covalently attached when they are hybridized to the target sequence. The presence of the covalently attached first and second sequences is related to the presence of the target polynucleotide sequence. The invention may be applied to target polynucleotide sequences in DNA or RNA. Specific target polynucleotide sequences of interest will frequently be characteristic of particular microorganisms, viruses, viroids, or genetic characteristics, including genetic abnormalities.

Claims2

Dependent claims

A kit for use in detecting a target polynucleotide sequence, which comprises in packaged combination:

a first polynucleotide complementary to and capable of hybridizing to a portion of a single strand of a target polynucleotide and having, or capable of having, means for immobilizing said first polynucleotide,
a second polynucleotide, having, or capable of having, a label wherein said second polynucleotide is complementary to and capable of hybridizing to a portion of said single strand of said target polynucleotide other than and non-contiguous with the portion complementary to said first polynucleotide, and
means for covalently attaching said first and second polynucleotides when said first and second polynucleotides are hybridized with said single strand of said target polynucleotide.

Dependencies are inferred from the claim text.

Facts

Application

08/401,660Filed 10 Mar 1995 · continuation

Priority

25 Aug 1988

Granted

14 May 1996

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C07H 21/00
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate
C12Q 1/6816
Hybridisation assays: characterised by the detection means
C12Q 1/6834
Hybridisation assays: enzymatic or biochemical coupling of nucleic acids to a solid phase
C12Q 1/6876
Involving nucleic acids: nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Family

Serial236,967Parent of this continuation · filed 25 Aug 1988

Serial993,156Parent of this continuation · filed 18 Dec 1992

Serial200,373Parent of this continuation · filed 18 Feb 1994

US 5,185,243 AParent of this continuation

US 5,516,641 AThis patent

From the US filing history. Foreign family members aren’t listed.

Method for detection of specific nucleic acid sequences

Life of this patent

Method for detection of specific nucleic acid sequences

Life of this patent

Abstract

Claims2