Antibodies specific for human prostate glandular kallkrein
AssigneeMayo Foundation for Medical Education and Research
InventorsDonald J. Tindall, Charles Y. F. Young, George G. Klee
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Abstract
The present invention provides an isolated preparation of monoclonal or polyclonal antibodies which react specifically with human prostate-specific glandular kallikrein (hK2), but which do not cross-react with human prostate-specific antigen (PSA), as well as immunogenic hK2 antigens useful to provide such antibodies.
Claims13
A method for detecting or determining hK2 in a sample of a human physiological fluid containing hK2 which comprises:
- (a) providing, attached to a solid surface, an amount of purified antibodies which specifically react with human prostate specific glandular kallikrein (hK2), wherein said antibodies do not significantly react with prostate-specific serum antigen (hK3);
- (b) contacting said antibodies on said solid surface with the sample to be tested for a sufficient time to allow the formation of binary complexes between at least a portion of said antibodies and a portion of said hK2;
- and
- (c) detecting or determining the presence or amount of hK2 complexed with said antibodies on said solid surface.
A preparation of polyclonal antibodies having the reactivity with hK2 of ATCC 97079.
A purified antibody which specifically reacts with human prostate specific glandular kallikrein (hK2), wherein said antibody does not significantly react with prostate-specific serum antigen (hK3).
A purified antibody which specificaHy reacts with a polypeptide of the formula:
- His--Cys--Leu--Lys--Lys--Asn--Ser--Gln--Val--Trp--Leu--Gly--Arg--His--Asn-- Leu (SEQ ID NO:1)
- or a subunit thereof, wherein said antibody does not significantly react with prostate specific antigen (hK3).
Dependencies are inferred from the claim text.