Methods of detecting hepatitis A virus
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsJohn R. Ticehurst, David Baltimore, Stephen M. Feinstone, Robert H. Purcell, Vincent R. Racaniello, Bahige M. Baroudy
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Abstract
Methods for producing HAV cDNA, products thereof, and uses thereof, are described. HAV cDNA is produced, for example, by reverse transcribing HAV RNA and subsequently inserting the HAV cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimetic plasmids containing the HAV cDNA. Such HAV cDNA is useful in assaying for the presence of HAV and in the production of HAV antigen and in the production of antibodies against HAV.
Claims13
An assay for the detection of hepatitis A virus in an RNA sample, said assay comprising:
- a. incubating said RNA sample with a labeled hepatitis A virus cDNA sequence under conditions which permit specific hybridization of said cDNA to said sample, said cDNA containing a sequence selected from the group consisting of clones pHAV.sub.LB 113, pHAV.sub.LB 1307, pHAV.sub.LB 228, pHAV.sub.LB 148 and pHAV.sub.LB 207 where said clones.
- b. separating unbound labeled cDNA from said sample;
- and
- c. detecting any remaining labeled cDNA.
An assay for detecting hepatitis A virus comprising a hepatitis A virus cDNA sequence containing a sequence selected from the group consisting of clones pHAV.sub.LB 113, pHAV.sub.LB 1307, pHAV.sub.LB 228, pHAV.sub.LB 148 and pHAV.sub.LB 207.
Dependencies are inferred from the claim text.