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AssigneesPurdue Research Foundation, Methodist Hospital of Indiana, Inc.
InventorsStephen F. Badylak, Robert J. Demeter, Michael Hiles, Sherry Voytik, Peter M. Knapp, Jr.
Estimating the term…
A fluidized, injectable tissue graft composition is described. The composition comprises comminuted intestinal submucosa or protease-digested intestinal submucosa. Methods for the preparation and use of injectable tissue graft compositions are described. In preferred embodiments the tissue graft material is prepared from the intestinal submucosa comprising the tunica submucosa, the muscularis mucosa and the stratum compactum of a segment of intestinal tissue of a warm-blooded vertebrate. Effective amounts of the fluidized graft compositions can be injected to promote repair tissue defects by inducing formation of endogenous tissues.
A method of preparing a tissue graft composition, said method comprising the steps of comminuting intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising intestinal submucosa delaminated from both the tunlea muscularis and at least the luminal portion of the tunica mucosa, and hydrating the resultant comminuted intestinal tissue to provide said tissue graft composition as a fluid having a viscosity of about 2 to about 300,000 cps at 25.degree. C.
A method of preparing a tissue graft composition, said method comprising the step of digesting intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, in aqueous solution with a protease for a period of time sufficient to solubilize said intestinal tissue to form a substantially homogeneous solution.
A composition comprising a suspension of comminuted intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising the tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, in an aqueous medium.
A composition comprising protease digested intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising the tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, in an aqueous carrier.
A method of promoting wound healing comprising the step of applying to a wound in need of healing an effective amount of a tissue graft composition comprising a suspension of comminuted intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, in an aqueous medium or a solution of protease digested intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, in an aqueous carrier.
Dried intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, said tissue being in powder form.
Dried protease-solubilized intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, said tissue being in powder form.
A method for inducing the formation of endogenous tissue of a warm-blooded vertebrate in a locale at which formation of endogenous tissue is desired, said method comprising providing to the locale a tissue graft composition comprising a suspension of comminuted intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, or a protease digest of said intestinal tissue in an amount effective to induce endogenous tissue growth in the locale.
A method of augmenting sphincter function in a warm-blooded mammal, the method comprising the step of injecting into tissue forming said sphincter an effective amount of a tissue graft composition comprising a suspension of comminuted intestinal tissue of a warm-blooded vertebrate, said intestinal tissue comprising tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa, or a protease digest of said intestinal tissue.
Dependencies are inferred from the claim text.