Injectable non-immunogenic cartilage and bone preparation
AssigneeChildren's Medical Center Corporation
InventorsAnthony Atala, Samy Ashkar
Life of this patent
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Abstract
Ground bone or cartilage particles are demineralized by extraction with a low ionic strength buffer such as 20 mM HEPES containing a chelating agent and protease inhibitors, then extracted with an acidic solution such as 0.3M citric acid, pH 4.0, containing protease inhibitors. The extracted material generally contains less than 2% by weight phosphate and less than 100 mM calcium. The phosphate content can be further reduced by treatment of the matrix with acid phosphatase, which removes residual organic phosphate. The material is useful in a method of treatment of vesicouretal reflux and other disorders where a bulking agent is effective is correcting the defect.
Claims19
A method for correcting a tissue defect without the creation of new tissue comprising administering to a patient in need of treatment thereof a demineralized organic matrix in an amount effective to correct the tissue defect without creation of new tissue, wherein the organic matrix is prepared by
- grinding growing bone or cartilage to form particles having a diameter of between approximately 80 and 200 microns,
- leaching the calcium and other divalent ions from the growing bone or cartilage with a buffered aqueous chelating solutionat at a temperature less than 15.degree. C. to produce a leached solid,
- extracting the leached solid with an acidic solution containing protease inhibitors at a temperature less than 15.degree. C.,
- further extracting the leached, extracted solid with a 1M salt solution to produce an organic matrix containing less than 2% by weight of phosphate and less than 100 mM calcium.
A method for preparing a demineralized organic matrix for correcting a tissue defect without creation of new tissue from growing bone or cartilage comprising
- grinding growing bone or cartilage to form particles having a diameter of between approximately 80 and 200 microns,
- leaching the calcium and other divalent ions from the growing bone or cartilage with a buffered aqueous chelating solution at at a temperature less than 15.degree. C. to produce a leached solid,
- extracting the leached solid with an acidic solution containing protease inhibitors at a temperature less than 15.degree. C., and
- further extracting the extracted, leached solid with a 1M salt solution at a temperature of less than 15.degree. C. to produce a demineralized organic matrix containing less than 2% by weight of phosphate and less than 100 mM calcium.
A demineralized organic matrix for correcting a tissue defect without creation of new tissue prepared by the method comprising
- grinding growing bone or cartilage to form particles having a diameter of between approximately 80 and 200 microns,
- leaching the calcium and other divalent ions from the growing bone or cartilage with a buffered aqueous chelating solution at at a temperature less than 15.degree. C. to produce a leached solid,
- extracting the leached solid with an acidic solution containing protease inhibitors at a temperature less than 15.degree. C., and
- further extracting the extracted, leached solid with a 1M salt solution at a temperature of less than 15.degree. C. to produce a demineralized organic matrix containing less than 2% by weight of phosphate and less than 100 mM calcium.
Dependencies are inferred from the claim text.