A method for protection of a plant from damage caused by an insect pest selected from the group of insects consisting of the order Lepidoptera and Diptera, comprising feeding the insect pest with the insecticidal crystal toxin produced by the strain Bacillus thuringiensis var. kurstaki No. 145 FERM BP-3905 (Bt. k 145), Bacillus thuringiensis var. kurstaki No. 161 FERM BP-3906, (Bt. k 161), and Bacillus thuringiensis var. kurstaki No. 116 FERM BP-3907 (Bt. k 116), wherein the insecticidal crystal toxin has a set of molecular weights as determined by 10% SDS-polyacrylamide gel electrophoresis of about 125,000 daltons and about 60,000 daltons when produced by Bt. k 145, about 130,000 daltons and about 60,000 daltons when produced by Bt. k 161, and about 130,000 daltons and about 60,000 daltons when produced by Bt. k 116.