Derivatives of human haemoglobin, method for preparing them, products derived therefrom and use of these derivatives and products
AssigneePasteur Merieux Serums et Vaccins
InventorsEdith Dellacherie, Daniel Sacco, Michel Grandgeorge
Life of this patent
Estimating the term…
Abstract
The invention relates, as new compounds, to derivatives of tetrameric human haemoglobin corresponding to the covalent coupling of a haemoglobin tetramer and a polycarboxy-substituted compound having at least 4 -COO.sup.- groups, the said polycarboxy-substituted compound effecting an internal crosslinking of the two dimers of haemoglobin and conferring thereon an affinity for oxygen which is compatible with an in vivo use of the conjugate when the natural allosteric effector of haemoglobin is not present. Among carboxy-substituted compounds, special mention is made of benzenehexacarboxylate and benzenetetracarboxylate.
Claims14
A tetrameric human haemoglobin for delivering oxygen to tissues and to the human body, formed by the covalent coupling of an oxyhaemoglobin tetramer and a polycarboxy-substituted compound, selected from the group consisting of tetracarboxy-substituted compounds and hexacarboxy-substituted compounds, the said polycarboxy-substituted compound being saturated or unsaturated monocylic molecules or branched or unbranched linear molecules having 4 to 6 carbons in the principal chain and effecting an internal crosslinking of the two dimers of haemoglobin and conferring thereon an affinity for oxygen.
A method for preparing a tetrameric human haemoglobin comprising the step of covalently cross-linking a tetrameric human oxyhaemoglobin in an aqueous solution with a polycarboxy-substituted non-polymeric compound having at least four COO.sup.- groups.
Dependencies are inferred from the claim text.