Antibiotic GE 2270 factors C.sub.2a

Antibiotic GE 2270 factor C.sub.2a having the following characteristics:

• A) Ultraviolet absorption spectrum exhibiting the following absorption maxima:
• ______________________________________ Solvent UV max (nm) ______________________________________ 0.1 M HCl 245-250 (shoulder) 300-315 0.1 M KOH 245-250 (shoulder) 300-315 Phosphate buffer pH 7.38 245-250 (shoulder) 300-315 Methanol 245-250 (shoulder) 300-315 ______________________________________
• B) .sup.1 H-NMR spectrum exhibiting the following groups of signals in DMSO-d.sub.6 (hexadeuterodimethylsulfoxide) using TMS as the internal standard (0.00 ppm) .delta., ppm, m! (s=singlet, d=doublet, t=triplet, m=multiplet, Py=pyridine, Tz=thiazole) 9.03, d, (NS);
• 8.70, d, (2NH's);
• 8.60, s, 8.54, s, 8.29, s, and 7.38, s, (Tz CH's);
• 8.48, m, (glycine NH);
• 8.43, d, and 8.27, d, (Py CH's);
• 7.35-7.20, m, (aromatic CH's and primary amide NH);
• 6.98, s (primary amide NH);
• 6.04, d, (OH);
• 5.80, t (OH);
• 5.35-5.15, m, (.alpha.CH's);
• 5.04, m, (phenylserine .beta.CH);
• 4.98, s CH.sub.2 (OCH.sub.3)!;
• 4.87, d, CH.sub.2 (OH)!;
• 4.81, m and 4.56, m, (oxazoline CH.sub.2);
• 4.35-3.75, m, (CH.sub.2 of glycine and prolineamide CH's);
• 3.39,s, (OCH.sub.3);
• 2.71, m, and 1.30, m, (CH.sub.2 of asparagine);
• 2.48, d, (NCH.sub.3 of N-methylasparagine);
• 2.22-1.80, m, (isopropyl CH and prolineamide CH's);
• 0.88 and 0.84, d, (valine CH.sub.3 's);
• C) Retention time (R.sub.t) of 12.6 min and retention time relative to antibiotic GE 2270 factor A (R.sub.t 16.6 min) of 0.76 in the following reverse phase HPLC system:
• Column:
• Bakerbond.RTM. C8 (5 .mu.m) 4.6.times.250 mm Flow rate:
• 1.8 ml/min
• Phase A:
• CH.sub.3 CN:tetrahydrofuran:40 mM HCOONH.sub.4 40:40:20
• Phase B:
• CH.sub.3 CN:tetrahydrofuran:40 mM HCOONH.sub.4 10:10:80
• Elution:
• linear gradient from 20% to 30% of Phase A in 20 min;
• Detection:
• UV 254 nm
• D) Main FAB-MS peak corresponding to the lowest isotope of the protonated molecular ion exhibiting a value of 1306 daltons when obtained on a Kratos MS-50 double focusing mass spectrometer, using 8 kV accelerating voltage and a saddle field atom gun with Xe gas (2.times.10.sup.-5 tort pressure indicated on the source ion guage) at 6 kV voltage and 1 mA current, the sample being mixed with a thioglycerol matrix containing 0.1M acetic acid;
• and its addition salts.