Antibodies against highly conserved amino acid sequences of insulin a process for the preparation of these antibodies and the use thereof in immunoassays
AssigneeHoechst Aktiengesellschaft
InventorStefan Mullner
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Abstract
Antibodies against highly conserved amino acid sequences of immunogenic substances, a process for the preparation of these antibodies and the use thereof in immunoassays. The invention relates to antibodies which are obtained by immunization with a peptide fragment which represents a highly conserved amino acid sequence of a native protein. The antibodies according to the invention can be used for the preparation of immunoassays, in particular for the preparation of assays for the determination of genetically engineered products such as insulin which arise as sparingly soluble inclusion bodies in microorganisms. The invention particularly relates to a multispecies insulin assay in the form of an RIA.
Claims13
An immunoassay method for the detection of an insulin or insulin derivative, comprising the steps of:
- (i) contacting said insulin or insulin derivative with one or more antibodies, wherein said antibody or antibodies are obtained by immunization with a peptide fragment selected from the group consisting of Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn and Leu-Glu-Asn-Tyr-Cys-Asn of the insulin A chain, and
- (ii) detecting the amount of said insulin or insulin derivative which binds to said antibody or antibodies.
An immunoassay kit comprising one or more antibodies, wherein said antibody or antibodies are obtained by immunization with a peptide fragment selected from the group consisting of Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn and Leu-Glu-Asn-Tyr-Cys-Asn of the insulin A chain.
Dependencies are inferred from the claim text.