Method and composition for testing blood for hemoglobin S that incorporates mineral oil as an insoluble upper phase
AssigneeMedicus Technologies, Inc.
InventorsDale Schoener, Gwen Parkin
Life of this patent
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Abstract
A method for testing blood for the presence of hemoglobin S wherein a blood sample is vigorously admixed with a two-phase test system for the purpose of determining the presence or absence of hemoglobin S after separation of the phases and wherein the two-phase test system involves an aqueous phase containing a lysing agent, a chemical reducing system and a phosphate ion buffer system; and an immiscible phase of mineral oil. The hemoglobin S presence is determinable by the color of a band formed at the interface of the aqueous and immiscible phases and wherein the invention further includes a composition suitable for testing whole blood for hemoglobin S.
Claims8
A method for testing blood for the presence of hemoglobin S comprising vigorously admixing a blood sample with a two-phase test system and determining the presence or absence of hemoglobin S after separation of the phases, the two-phase system comprising:
- (a) an aqueous phase containing a lysing agent, a chemical reducing system and a phosphate ion buffer system;
- and
- (b) an immiscible phase consisting essentially of mineral oil;
- the hemoglobin S presence being determinable by the color of a band formed at the interface of the aqueous and immiscible phases.
A composition suitable for testing whole blood for hemoglobin S comprising:
- (a) an aqueous phase containing a lysing agent, a chemical reducing system and a phospate ion buffer system;
- and
- (b) an immiscible phase consisting essentially of mineral oil.
A kit suitable for testing whole blood for hemoglobin S comprising:
- (a) an aqueous phase containing a lysing agent, a chemical reducing system and a phosphate ion buffer system;
- and
- (b) an immiscible phase consisting essentially of mineral oil.
Dependencies are inferred from the claim text.