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US 5,514,587 AGranted
DNA fragment encoding a hydrogen peroxide-generating NADH oxidase
AssigneeNippon Paint Co., Ltd.
InventorsMasako Higuchi, Junichi Matsumoto, Yoshikazu Yamamoto, Yoshiyuki Kamio, Kazuo Izaki
Life of this patent
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Abstract
A gene encoding a hydrogen peroxide-generating NADH oxidase and a method for preparing a large amount of the NADH oxidase with the use of the gene and gene recombinant techniques are disclosed.
Claims5
A DNA molecular comprising nucleotides 279 through 1809 of sequence I.D. No. 1, wherein the molecule has the following base sequence:
- ______________________________________ AT GGCATTAGAC GCAGAAATCA AAGAGCAGTT AGGACAGTAT CTTCAATTAC TTGAGTGTGA GATTGTTTTA CAAGCTCAAT TAAAAGACGA TGCTAATTCT CAAAAAGTTA AGGAATTTCT CCAAGAAATC GTTGCAATGT CTCCTATGAT TTCTTTAGAC GAAAAGGAAC TTCCGCGAAC ACCTAGTTTT CGCATAGCTA AAAAGGGGCA AGAATCTGGT GTTGAATTTG CTGGCTTACC CCTTGGTCAC GAATTTTACT TCGTTTATCT TGGCTCTGTT ACAGGTTTCA GGGCGTCGGC TAAGGTAGAG ACTGATATTG TCAAACGCAT TCAAGCTGTT GATGAACCTA TGCATTTTGA AACCTATGTT AGTTTGACTT GTCATAATTG TCCAGATGTT GTTCAGGCTT TCAATATCAT GTCAGTTGTT AATCCCAACA TTTCACATAC AATGGTGGAA GGTGGCATGT TTAAAGATGA AATTGAAGCT AAGGGAATTA TGTCTGTGCC AACTGTCTAT AAAGATGGAA CAGAATTTAC CTGAGGGCGT GCTAGCATAG AGCAATTACT AGACTTGATA GCAGGTCCTG TTAAAGAAGA TGCTTTTGAT GATAAAGGTG TTTTTGATGT CCTTGTTATT GGTGGGGGTC CTGCTGGTAA TAGCGCGGCT ATCTATGCTG CAAGAAAGGG AGTTAAAACA GGACTTTTAG CTGAAACCAT GGGTGGTCAA GTTATGGAAA CCGTGGGTAT TGAAAATATG ATCGGTACCC CATATGTTGA AGGACCCCAA TTAATGGCTC AGGTGGAAGA GCATACCAAG TCTTATTCTG TTGACATCAT GAAGGCACCG CGTGCTAAGT CTATTCAAAA GACAGACTTG GTTGAAGTTG AACTTGATAA TGGAGCTCAT TTGAAAGCAA AGACAGCTGT TTTGGCCTTA GGTGCCAAGT GGCGTAAAAT CAATGTACCA GGAGAAAAAG AATTCTTTAA TAAAGGTGTT ACTTACTGTC CGCACTGTGA TGGTCCTCTT TTCACAGACA AAAAAGTCGC TGTCATTGGC GGTGGAAACT CAGGTTTAGA AGCAGCTATT GATTTGGCTG GGTTAGCTAG CCATGTCTAT ATTTTAGAAT TTTTACCTGA GTTAAAAGCT GATAAGATCT TACAAGATCG TGCGGAAGCT CTTGATAATA TTACCATTGT AACTAATGTT GCGACTAAAG AAATTATTGG CAATGACCAC GTAGAAGGTC TTCGTTACAG TGATCGTACG ACCAATGAAG AGTACTTGCT TGATTTAGAA GGTGTTTTTG TTCAAATTGG ATTGGTACCT AGTACTGACT GGTTAAAGGA TAGTGGACTA GGACTCAATG AAAAAGGTGA AATCATTGTT GCTAAAGATG GCGCAACTAA TATTCCTGCT ATTTTTGCAG CTGGTGATTG CACAGATAGT GCCTACAAAC AAATTATCAT TTCCATGGGT TCTGGAGCTA CTGCGGCTTT AGGTGCCTTT GATTATTTGA TTAGAAATT/. ______________________________________
A DNA molecule comprising the amino acid sequence I.D. No. 2, wherein the molecule encodes the following amino acid sequence:
- __________________________________________________________________________ Met Ala Leu Asp Ala Glu Ile Lys Glu Gln Leu Gly Gln Tyr Leu 15 Gln Leu Leu Glu Cys Glu Ile Val Leu Gln Ala Gln Leu Lys Asp 30 Asp Ala Asn Ser Gln Lys Val Lys Glu Phe Leu Gln Glu Ile Val 45 Ala Met Ser Pro Met Ile Ser Leu Asp Glu Lys Glu Leu Pro Arg 60 Thr Pro Ser Phe Arg Ile Ala Lys Lys Gly Gln Glu Ser Gly Val 75 Glu Phe Ala Gly Leu Pro Leu Gly His Glu Phe Tyr Phe Val Tyr 90 Leu Gly Ser Val Thr Gly Phe Arg Ala Ser Ala Lys Val Glu Thr 105 Asp Ile Val Lys Arg Ile Gln Ala Val Asp Glu Pro Met His Phe 120 Glu Thr Tyr Val Ser Leu Thr Cys His Asn Cys Pro Asp Val Val 135 Gln Ala Phe Asn Ile Met Ser Val Val Asn Pro Asn Ile Ser His 150 Thr Met Val Glu Gly Gly Met Phe Lys ASp Glu Ile Glu Ala Lys 165 Gly Ile Met Ser Val Pro Thr Val Tyr Lys Asp Gly Thr Glu Phe 180 Thr Ser Gly Arg Ala Ser Ile Glu Gln Leu Leu Asp Leu Ile Ala 195 Gly Pro Leu Lys Glu Asp Ala Phe Asp Asp Lys Gly Val Phe Asp 210 Val Leu Val Ile Gly Gly Gly Pro Ala Gly Asn Ser Ala Ala Ile 225 Tyr Ala Ala Arg Lys Gly Val Lys Thr Gly Leu Leu Ala Glu Thr 240 Met Gly Gly Gln Val Met Glu Thr Val Gly Ile Glu Asn Met Ile 255 Gly Thr Pro Tyr Val Glu Gly Pro Gln Leu Met Ala Gln Val Glu 270 Glu His Thr Lys Ser Tyr Ser Val Asp Ile Met Lys Ala Pro Arg 285 Ala Lys Ser Ile Gln Lys Thr Asp Leu Val Glu Val Glu Leu Asp 300 Asn Gly Ala His Leu Lys Ala Lys Thr Ala Val Lue Ala Lue Gly 315 Ala Lys Trp Arg Lys Ile Asn Val Pro Gly Glu Lys Glu Phe Phe 330 Asn Lys Gly Val Thr Tyr Cys Pro His Cys Asp Gly Pro Leu Phe 345 Thr Asp Lys Lys Val Ala Val Ile Gly Gly Gly Asn Ser Gly Leu 360 Glu Ala Ala Ile Asp Leu Ala Gly Leu Ala Ser His Val Tyr Ile 375 Leu Glu Phe Leu Pro Glu Leu Lys Ala Asp Lys Ile Leu Gln Asp 390 Arg Ala Glu Ala Lue Asp Asn Ile Thr Ile Leu Thr Asp Val Ala 425 Thr Lys Glu Ile Ile Gly Asn Asp His Val Glu Gly Leu Arg Tyr 420 Ser Asp Arg Thr Thr Asn Glu Glu Tyr Leu Leu Asp Leu Glu Gly 435 Val Phe Val Gln Ile Gly Leu Val Pro Ser Thr Asp Trp Leu Lys 450 Asp Ser Gly Leu Ala Leu Asn Glu Lys Gly Glu Ile Ile Val Ala 465 Lys Asp Gly Ala Thr Asn Ile Pro Ala Ile Phe Ala Ala Gly Asp 480 Cys Thr Asp Ser Ala Tyr Lys Gln Ile Ile Ile Ser Met Gly Ser 495 Lys Ala Thr Ala Ala Leu Gly Ala Phe Asp Tyr Leu Ile Arg Asn 510. __________________________________________________________________________
A DNA molecule wherein the 5' terminal non-translated region comprises the following base sequence:
- __________________________________________________________________________ GGATCCCTTC TCATGTTCTC TCACAAGGAT TTGAGGTTTT AGGTGAAGAT GGTTTAGCAC 60 AACGTGGAAC CTTTATTGTA GATCCGGATG GTATCATTCA AATGATGGAA GTCAATGCAG 120 ATGGTATTGG TCGTGATGCT ACTACCTTGA TTGATAAAGT TGTGCAGCT CAATCTATTC 180 GCCAACATCC AGGAGAAGTT TGCCCTGCCA AATGGAAAGA GGGAGCTGAA ACTTTAAAAC 240 CAAGTTTGGT ACTTGTCGGT AAAATTTAAG GAGAAACT. __________________________________________________________________________
Dependencies are inferred from the claim text.