Cloning of cellulase genes from acidothermus cellulolyticus
AssigneeMidwest Research Institute
InventorsStanley M. Lastick, deceased, Melvin P. Tucker, Karel Grohmann
Life of this patent
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Abstract
A process is described for moving fragments that code for cellulase activity from the genome of A. cellulolyticus to several plasmid vectors and the subsequent expression of active cellulase acitivty in E. coli.
Claims8
A DNA fragment coding for cellulase enzymes which comprises about 2.0 kilobase pairs and is described by a restriction map shown in FIG. 2-1;
- wherein said DNA fragment is isolated from Acidothermus cellulolyticus.
A DNA fragment coding for cellulase enzymes which comprises about 3.7 kilobase pairs and is described by a restriction map shown in FIG. 2--2;
- wherein said DNA fragment is isolated from Acidothermus cellulolyticus.
A transformed microorganism carrying a recombinant plasmid comprising a DNA fragment coding for cellulase enzymes, wherein said DNA fragment comprises approximately 2.0 kilobase pairs which is described by the restriction map shown in FIG. 2-1, wherein said DNA fragment is isolated from Acidothermus cellulolyticus, wherein said transformed microorganisms comprises E. coli bacterium strain DH5.alpha. or strain HB101.
A transformed microorganism carrying a recombinant plasmid comprising a DNA fragment coding for cellulase enzymes, wherein said DNA fragment comprises approximately 3.7 kilobase pairs which is described by the restriction map shown in FIG. 2--2, wherein said DNA fragment is isolated from Acidothermus cellulolyticus, wherein said transformed microorganism comprises E. coli bacterium strain DH5.alpha..
Dependencies are inferred from the claim text.