US 5,514,580 A · In vitro leaf petiole multiplication of pelargoniums

Abstract

A process for propagating Pelargonium x domesticum varieties by tissue culture propagation of petiole sections taken from a mother plant. In addition to tissue culture propagation steps and growth media constituents which are known in the art, an essential step of the preferred embodiment of the invention includes the conducting of callus formation by initial culturing of the petiole section in the dark. In all embodiments of the invention, however, it is believed that the effectiveness of the present process is attributable at least in part to the presence in the culture medium of a growth regulator selected from the group consisting of amino or benzyl-glucosides and amino- or benzyl-glycosides or any other composition chemically equivalent to the exemplary regulator benzylamino riboside.

Claims7

Dependent claims

A method for propagating a Pelargonium x domesticum mother plant, comprising the steps of:

a) harvesting one or more petioles from a Pelargonium x domesticum mother plant;
b) culturing solely petiole tissue from the petioles harvested in step a) in a culture medium, said culture medium containing benzylaminoriboside, in the dark;
c) exposing said petiole tissue from step b) to periods of light and darkness;
d) removing small plants from a clump of small plants resulting from the culturing of said petiole tissue;
and
e) rooting and growing said small plants, whereby substantially uniform plants are produced.

3 more elements

Dependencies are inferred from the claim text.

In vitro leaf petiole multiplication of pelargoniums

Life of this patent

In vitro leaf petiole multiplication of pelargoniums

Life of this patent

Abstract

Claims7