Human transglutaminases
AssigneeZymoGenetics, Inc.
InventorsPatrick J. O'Hara, Francis J. Grant, Paul O. Sheppard
Life of this patent
Estimating the term…
Abstract
Human prostatic and placental transglutaminases are identified and cloned. The human transglutaminases herein are useful for, inter alia, therapeutic wound repair, closure of skin grafts, stabilizing food preparations, and markers for identifying agents which act as agonists or antagonists of cellular apoptosis.
Claims15
An isolated polynucleotide molecule which codes for human prostatic transglutaminase comprising the amino acid sequence of SEQ ID NO:14 and allelic variants thereof.
An isolated polynucleotide molecule which codes for human placental transglutaminase, wherein the molecule comprises the nucleotide sequence of human placental transglutaminase of Seq. ID No. 22.
A DNA construct for the expression of human prostatic transglutaminase, which comprises the following operably linked elements:
- a transcriptional promoter.;
- a DNA molecule encoding a human prostatic transglutaminase polypeptide comprising the amino acid sequence of SEQ ID NO:14 and allelic variants thereof;
- and
- a transcriptional terminator.
A probe which comprises an oligonucleotide of at least about 14 nucleotides capable of specifically hybridizing with a gene which encodes a human prostatic or placental transglutaminase polypeptide, wherein said probe is at least 85% homologous to a sequence of the human prostatic or placental transglutaminase of Seq. ID. No. 14 or Seq. ID. No. 22 or its complement.
A DNA construct for the expression of human placental transglutaminase, which comprises the following operably linked elements:
- a transcriptional promoter;
- a DNA molecule encoding a human placental transglutaminase polypeptide comprising the nucleotide sequence of SEQ ID NO:22 and allelic variants thereof;
- and a transcriptional terminator.
Dependencies are inferred from the claim text.