Polynucleotides encoding insect steroid hormone receptor polypeptides and cells transformed with same
AssigneeThe Board of Trustees of the Leland Stanford University
InventorsDavid S. Hogness, Michael R. Koelle, William A. Segraves
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Abstract
Polynucleotide sequences which encode ecdysone receptors have been isolated and expressed in host cells.
Claims8
An isolated polynucleotide encoding a Drosophila ecdysone receptor having the 878 amino acid predicted amino acid sequence of FIG. 4 (A-C) and which has ecdysteroid-binding activity wherein said ecdysone receptor exhibits saturable binding to .beta.-ecdysone or ecdysone analogues.
A transformed insect bacterial or mammalian host cell comprising a polynucleotide encoding a Drosophila ecdysone receptor consistinq of the 878 amino acid predicted amino acid sequence of FIG. 4 (A-C) operably linked to a promoter, wherein said promoter is not naturally associated with an ecdysone receptor gene in the germline of naturally occurring Drosophila.
Dependencies are inferred from the claim text.