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US 5,514,560 AGranted
Substrates for .beta.galactosidase
AssigneeBoehringer Mannheim GmbH
InventorsWayne B. Manning, Pyare Khanna, Glenda Choate
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Abstract
Galactopyranoside derivative compounds for use as substrates for hydrolysis by enzymes with .beta.-galactosidase activity are provided. The concentration of the reaction products can be measured by spectroscopy. The compounds find particular use in diagnostic assays for detection of analyte.
Claims6
In a method for determining the .beta.-galactosidase enzyme activity of a medium, the improvement which comprises:
- using as the enzyme substrate a compound for use as a substrate by enzymes with .beta.-galactosidase activity, said compound having the formula:
- ##STR2## wherein:
- R is .beta.-D-galactosidyl;
- A is selected from the group consisting of --OCH.sub.3 and --CHO, when B is H;
- and
- B is selected from the group consisting of --NO.sub.2 and --CN, when A is H;
- and
- one of A and B is H.
5 more elements
Dependencies are inferred from the claim text.