Method and kit for detecting antibodies specific for HLA and/or platelet glycoproteins
AssigneeGenetic Testing Institute Inc.
InventorMichael Moghaddam
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Abstract
The present invention is directed to determining the presence of antibodies from a patient's blood which are specific for HLA Class I antigens and/or platelet glycoproteins. The invention provides for a method and kit for diagnosing platelet disorders in an easy to use format. The method and kit employ a solid support, such as microtiter plate wells, that are coated with the HLA and glycoprotein antigens. A sample of the patient's blood is added to the coated wells and allowed to react, then spectrophotometric detection of antibodies provides diagnostic quantitation.
Claims15
A method for detecting and identifying the presence of antibodies from a patient's blood specific for purified HLA which are differentiated from antibodies specific for purified platelet glycoproteins comprising:
- a. obtaining serum from the patients blood;
- b. placing the serum on a solid support having one well with the purified HLA attached thereto and another well with the purified platelet glycoproteins attached thereto;
- c. detecting the presence of the antibodies on the solid support;
- d. identifying the antibodies detected in step c.
A kit for detecting and identifying the presence of antibodies from a patient's blood specific for purified HLA which are differentiated from antibodies specific for purified platelet glycoproteins comprising:
- a. a solid support having one well with the purified HLA attached thereto and another well with the purified platelet glycoproteins attached thereto;
- b. a receptacle containing an enzyme label solution for detecting the presence of the antibodies and identifying the antibodies detected.
Dependencies are inferred from the claim text.