US 5,514,546 AGranted

Stem-loop oligonucleotides containing parallel and antiparallel binding domains

AssigneeResearch Corporation Technologies, Inc.

InventorEric T. Kool

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present invention provides stem-loop oligonucleotides containing a double-stranded stem domain of at least about 2 base pairs and a single-stranded loop domain. The loop domains of the present oligonucleotides include at least one parallel binding (P) domain separated by at least about 3 nucleotides from a corresponding anti-parallel binding (AP) domain. Each P and corresponding AP domain of the present oligonucleotides can bind detectably to one strand of a defined nucleic acid target wherein the P domain binds in a parallel manner to the target and the corresponding AP domain binds in an anti-parallel manner to the target. The present stem-loop oligonucleotides can bind to both single-stranded and double-stranded target nucleic acids. The present invention also provides methods of using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.

Claims9

Dependent claims

A method of detecting a target nucleic acid which comprises:

contacting a sample to be tested for containing said target nucleic acid with a stem-loop oligonucleotide for a time and under conditions sufficient to form an oligonucleotide-target complex, wherein said stem-loop oligonucleotide comprises a double-stranded stem domain of at least about two base pairs and a single-stranded loop domain comprising at least one parallel binding (P) domain separated from at least one anti-parallel binding (AP) domain by a spacer having a length equivalent to at least about three nucleotides, wherein one P domain and one AP domain can simultaneously and detectably bind to one strand of said target nucleic acid, wherein said P domain is capable of binding in a parallel manner to said target by a sufficient number of Hoogsteen bonds to achieve detectable binding, and wherein said AP domain is capable of binding in an antiparallel manner to said target by a sufficient number of Watson-Crick bonds to achieve detectable binding;
and detecting said complex, wherein detection of said complex is indicative of the presence of said target nucleic acid.

Dependencies are inferred from the claim text.