Nucleotide and deduced amino acid sequences of the envelope 1 gene of 51 isolates of hepatitis C virus and the use of reagents derived from these sequences in diagnostic methods and vaccines
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsJens Bukh, Roger H. Miller, Robert H. Purcell
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Abstract
The nucleotide and deduced amino acid sequences of 51 cDNAs are disclosed where each cDNA encodes the envelope 1 gene of an isolate of hepatitis C virus (HCV). The invention relates to the oligonucleotides, peptides and recombinant envelope 1 proteins derived from these sequences and their use in diagnostic methods and vaccines.
Claims10
A method for detecting the presence of hepatitis C virus comprising:
- (a) amplifying RNA via reverse transcription-polymerase chain reaction to produce amplification products;
- (b) contacting said products with a nucleotide sequence selected from the sequences shown in SEQ ID NO:
- 1-51;
- and
- (c) detecting complexes of said products and said nucleotide sequence.
A method for detecting the presence of hepatitis C virus comprising:
- (a) amplifying RNA via a reverse transcription-polymerase chain reaction process using primers selected from the group consisting of SEQ ID NO.:
- 103 through SEQ ID NO. 108;
- and
- (b) detecting said amplification product.
Isolated primers, wherein said primers have nucleic acid sequences selected from the group consisting of SEQ ID NO:
- 103 through SEQ ID NO:
- 108.
A diagnostic kit for use in detecting the presence of hepatitus C virus, said kit comprising:
- primers having nucleic acid sequences selected from the group consisting of SEQ ID NO:103 through SEQ ID NO:108.
A method for determining the genotype of a hepatitis C virus, said method comprising:
- (a) amplifying RNA via reverse transcription-polymerase chain reaction to produce amplification products;
- (b) contacting said products with at least one E1 region genotype-specific oligonucleotide;
- and
- (c) detecting complexes of said products and said oligonucleotide(s).
Isolated genotype-specific oligonucleotides having nucleic acid sequences deduced from genotype-specific nucleic acid domains located in SEQ ID Nos:
- 1 through 51 or in consensus sequences produced from at least two sequences shown in SEQ ID Nos:
- 1 through 51.
Isolated genotype-specific oligonucleotides having nucleic acid sequences selected from the group consisting of SEQ ID NO:
- 109 through SEQ ID NO:
- 135.
A diagnostic kit for determining the genotype of a hepatitis C virus, said kit comprising primers selected from the group consisting of SEQ ID NO:103 through SEQ ID NO:108 and hybridization probes selected from the group consisting of SEQ ID NO:109 through SEQ ID NO:135.
Dependencies are inferred from the claim text.