Supercritical fluid extraction of cholesterol from liquid egg yolk
AssigneeThe Regents of the University of California
InventorsGideon Zeidler, Gonca Pasin, Annie J. King
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Abstract
A method for extraction of cholesterol from untreated yolk with supercritical carbon dioxide. The supercritical carbon dioxide extraction of cholesterol from the liquid egg yolk is performed in the presence or absence of cosolvents or other agents enhancing the cholesterol extraction. All agents are non-toxic and safe for human consumption.
Claims20
A method for supercritical fluid extraction of at least 55% of cholesterol by weight from intact untreated liquid egg yolk having about 50% moisture and pH 6.0-6.6, said method comprising steps:
- (a) contacting the untreated liquid egg yolk with a vegetable oil in a ratio from 1 volume of egg yolk to 0.5 to 4 volumes of oil;
- (b) homogenizing a mixture of step (a) at about 20,000 to 25,000 rpm for about 15-45 minutes;
- (c) extracting the homogenate of step (b) with supercritical carbon dioxide at an extraction pressure of between about 2,000 and 4,500 psig and at a temperature between 31.degree. C. and 45.degree. C., having a solvent to feed ratio from 50:1 to 100:1;
- and
- (d) separating the liquid egg yolk from the supercritical carbon dioxide containing extracted cholesterol and the vegetable oil used in step (a).
A method for supercritical fluid extraction of at least 55% of cholesterol by weight from intact untreated liquid egg yolk having pH between 6.0 and 6.6 and containing about 50% moisture, said method comprising steps:
- (a) contacting the untreated liquid egg yolk with soybean oil in a ratio about 1:2, volume/volume;
- (b) homogenizing a mixture of step (a) for about 15-45 minutes at 20,000-25,000 rpm;
- (c) extracting the homogenate of step (b) with supercritical carbon dioxide at extraction pressure about 3,500 psig and at temperature between 35.degree. C. and 40.degree. C. having a solvent to feed ratio from 50:1 to 100:1;
- (d) repeating step (c) one to five times;
- and
- (e) separating the liquid egg yolk from the supercritical carbon dioxide containing extracted cholesterol and the oil used in step (a).
A method for supercritical fluid extraction of at least 55% of cholesterol by weight from intact untreated liquid egg yolk containing about 50% moisture and having pH from 6.0 to 6.6, said method comprising steps:
- (a) contacting the untreated liquid egg yolk with soybean oil in a ratio of 1:2 volume/volume;
- (b) homogenizing a mixture of step (a) for about 15-45 minutes at 20,000-25,000 rpm;
- (c) extracting the homogenate of step (b) with supercritical carbon dioxide containing from 1% to 10% ethanol at extraction pressure about 3,500 psig and at temperature between 35.degree. C. and 40.degree. C. having a solvent to feed ratio of from 50:1 100:1;
- and
- (d) separating the liquid egg yolk from the supercritical carbon dioxide containing extracted cholesterol and the vegetable oil used in step (a).
A method for supercritical fluid extraction of at least 55% of cholesterol by weight from intact untreated liquid egg yolk containing about 50% moisture and having pH from 6.0 to 6.6, said method comprising steps:
- (a) contacting the untreated liquid egg yolk with soybean oil in a ratio of 1:2 volume/volume;
- (b) homogenizing a mixture of step (a) for about 15-45 minutes at 20,000-25,000 rpm;
- (c) extracting the homogenate of step (b) with supercritical carbon dioxide containing from 1% to 10% of methanol at extraction pressure about 3,500 psig and at temperature between 35.degree. C. and 40.degree. C. having a solvent to feed ratio of from 50:1 to 100:1;
- and
- (d) separating the liquid egg yolk from the supercritical carbon dioxide containing cholesterol extracted into the oil.
Dependencies are inferred from the claim text.