Flavivirus recombinant poxvirus vaccine
AssigneeVirogenetics Corporation
InventorsEnzo Paoletti, Steven E. Pincus
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Abstract
What is described is a recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, containing foreign DNA from flavivirus, such as Japanese encephalitis virus, yellow fever virus and Dengue virus. In a preferred embodiment, the recombinant poxvirus generates an extracellular particle containing flavivirus E and M proteins capable of inducing neutralizing antibodies, hemagglutination-inhibiting antibodies and protective immunity against flavivirus infection. What is also described is a vaccine containing the recombinant poxvirus for inducing an immunological response in a host animal inoculated with the vaccine.
Claims22
A recombinant vaccinia virus comprising DNA coding for Japanese encephalitis virus protein M or a precursor to protein M, and Japanese encephalitis virus proteins E, NS1 and NS2A, in a nonessential region of the vaccinia genome.
A recombinant vaccinia virus wherein regions C7L-K1L, J2R, B13R+B14R, A26L, A56R, and I4L have been deleted therefrom, and further comprising DNA from Japanese encephalitis virus in a non-essential region of the vaccinia genome.
A recombinant vaccinia virus wherein the open reading frames for the thymidine kinase gene, the hemorrhagic region, the A type inclusion body region, the hemagglutinin gene, the host range gene region, and the large subunit, ribonucleotide reductance have been deleted therefrom, and further comprising DNA from Japanese encephalitis virus in a non-essential region of the vaccinia genome.
A recombinant vaccinia virus comprising DNA from Japanese encephalitis virus in a nonessential region of the vaccinia genome wherein the DNA codes for a precursor to Japanese encephalitis virus protein M and Japanese encephalitis virus proteins C, E, NS1 and NS2A;
- or, the DNA codes for Japanese encephalitis virus proteins NS1 and NS2A;
- or, the DNA codes for Japanese encephalitis virus proteins NS1, NS2A and NS2B.
Dependencies are inferred from the claim text.