US 5,514,255 AGranted

Vertically stacked trays overflowing liquid congealing into electrophoresis gel, and optional combination spacer-combs between the trays

OwnerJames Gautschinventor

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Abstract

Multiple, typically 5+, trays of an identical special configuration are vertically stacked one atop the next, end-to-end reversed. Each tray has an overflow feature, normally in the form of either (i) a portion of a side that is reduced in height so as to form a lip, (ii) a corner hole protected by a removable baffle of selectable height, or (iii) a threaded standpipe that may be screwed to variable height. The overflow feature permits that liquid will overflow each tray only upon reaching a predetermined height in a reservoir of the tray. A liquid poured in the top tray cascades downwards from tray to tray, filling each tray to a uniform even level and ultimately reaching the bottom tray of the stack. The liquid that is within each tray is permitted to gel, forming thereby a gel state material that is suitable to receive samples upon which electrophoresis may be conducted. Combination spacer and toothed comb elements are optionally inserted between the stacked trays. These spacer-comb elements serve to (i) space apart the stacked trays so that any untoward and undesired wicking of liquid onto the underside of the tray is especially precluded, and to simultaneously (ii) place arrayed small slits, or wells, in the gel--into which wells samples, upon which electrophoresis is ultimately conducted, are inserted.

Claims7

Dependent claims

A method of preparing flat sheets of a gel state material suitable to receive samples upon which electrophoresis may be performed, the method comprising:

vertically stacking in a vertical stack
a plurality of trays each having (i) a base and sides defining a substantially flat substantially shallow interior reservoir, (ii) an overflow outlet from which liquid received into the reservoir will flow out of the reservoir when a predetermined liquid level is reached in the reservoir, and (iii) a top opening located so that it may be disposed in position below the overflow outlet of any immediately overlying tray in the vertical stack of trays, interleaved with
a plurality of spacers inserted between successive ones of the vertically stacked plurality of trays, each a spacer at least partially in the aspect of a comb having downwards extending teeth;
wherein, by the interleaved stacking of trays and of spacers having at least the partial aspect of a comb having downwards extending teeth, an array of small volumes in the reservoir of each tray that supports a spacer is protected by the teeth features of the spacer against any incursion of liquid;
pouring a liquid material that will gel to a gel state into an uppermost one of vertically stacked trays so that the liquid fills the reservoir of this uppermost tray to a predetermined height it will then overflow through the overflow outlet of this uppermost tray, flowing downwards into the reservoir of a next lower tray and successively cascading downwards from tray to tray as the reservoir of each tray is filled to a uniform even level until, ultimately, a reservoir of bottom one of the stacked trays is reached and filled with the liquid material;
letting the liquid material in each tray gel so as to form a gel state material suitable to receive samples upon which electrophoresis is conducted;
and
removing the at least one spacer upon the gelling of the liquid material, leaving an array of voids in the gel state material suitable to receive the samples upon which electrophoresis is ultimately conducted;
wherein a plurality of trays each containing flat sheets of a gel state material suitable to receive samples upon which electrophoresis may be performed have been simultaneously prepared.

6 more elements

A covered tray suitably used with a number of identical covered trays in order to prepare in each tray from a liquid material poured into a reservoir of the covered tray a flat sheet of a gel state material suitable to receive samples upon which electrophoresis may be performed, each covered tray comprising:

a tray body suitably vertically stacked with a number of other tray bodies in a vertical stack, the tray body having and defining a substantially flat interior reservoir, an overflow outlet from which liquid received into the reservoir will flow out of the reservoir when a predetermined liquid level is reached in the reservoir, and a top opening located so that it may be disposed in position below the overflow outlet of any immediately overlying tray body in the vertical stack of tray bodies;
a cover member at least partially overlying the reservoir of each tray body and to the extent so overlying the reservoir forming a cover thereto, the cover member having and presenting teeth in the manner of a comb which teeth extend, when the cover member is overlying the reservoir, into the reservoir, therein to establish within the reservoir an array of small volumes into which volumes any liquid poured into the reservoir cannot enter;
wherein, when a liquid material is poured into the tray body while the cover member is in place at least partially overlying the tray body's reservoir, and after the liquid material is allowed to gel to a gel state, a separation of the cover member from the tray body leaves an array of voids in the gel state material that is within the tray body's reservoir, the voids being suitable to receive samples upon which electrophoresis transpires.

Dependencies are inferred from the claim text.