Compositions useful in heterogeneous immunoassays
AssigneeSyntex (U.S.A.) Inc.
InventorsEdwin F. Ullman, Hriar Kirakossian, Mary C. Ericson, Richard P. Watts
Life of this patent
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Abstract
A method for carrying out an immunoassay for an analyte in which a sample suspected of containing an analyte and reagents useful for detecting the analyte of interest are combined in an aqueous medium, wherein one of the reagents includes a support and one includes a label, the improvement comprising employing as the reagents a first and second conjugate, each comprised of a specific binding pair (sbp) member bound to a small molecule wherein the small molecules of each conjugate are different.
Claims12
A composition of matter consisting of a conjugate of a monoepitopic antigen covalently bound to a small molecule, said conjugate being bound in a termolecular complex with antibody for said monoepitopic antigen and an antibody for said small molecule, wherein said small molecule is an organic or organometallic group having a molecular weight of from 100 to 2000 and for which a receptor exists or can be prepared.
A composition of matter
- E-Ab.sub.F :F-hapten:Ab.sub.hapten -X:
- Y-support
- where E-Ab.sub.F is an anti-fluorescein antibody bound to an enzyme;
- F-hapten is a fluorescein bound hapten;
- Ab.sub.hapten -X is an anti-hapten antibody bound to biotin;
- and Y-support is avidin bound to a support.
A composition of matter
- E-Ab.sub.F :F-Ab.sub.1 :A:Ab.sub.2 -X:Y-support
- where E-Ab.sub.F is an anti-fluorescein antibody bound to an enzyme;
- F-Ab.sub.1 is a fluorescein bound first antibody;
- A is a multiepitopic antigen;
- Ab.sub.2 -X is a second antibody bound to biotin;
- and Y-support is avidin bound to a support.
A composition of matter
- E-Ab.sub.F :F-Ag:Ab:Ab-X:Y-support
- where E-Ab.sub.F is an anti-fluorescein first antibody bound to an enzyme;
- F-Ag is a fluorescein bound antigen;
- Ab is a second antibody complementary to said antigen;
- Ab-X is a third antibody complementary to said second antibody bound to biotin;
- and Y-support is avidin bound to a support.
A composition of matter
- E-Ab.sub.F :F-Ab:Ab:Ag-X:
- Y-support
- where E-Ab.sub.F is an anti-fluorescein first antibody bound to an enzyme;
- F-Ab is a fluorescein bound antibody;
- Ab is a second antibody;
- Ag-X is an antigen complementary to said second antibody bound to biotin;
- and Y-support is avidin bound to a support.
A composition of matter
- E-Ab.sub.F :F-Ab.sub.1 :A:Ab.sub.2 -X
- where E-Ab.sub.F is an anti-F antibody bound to an enzyme, where F is a first small molecule;
- F-Ab.sub.1 is said first small molecule (F) covalently bound to a first antibody;
- A is a multiepitopic antigen;
- and Ab.sub.2 -X is a second antibody covalently bound to a second small molecule., wherein said small molecules are organic or organometallic groups having a molecular weight of from 100 to 2000 and for which a receptor exists or can be prepared.
Dependencies are inferred from the claim text.