Expression vectors responsive to steroid hormones
AssigneeMcGill University
InventorsSylvie Mader, John H. White
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Abstract
Expression vector adapted for expression of cloned genes in an animal cell comprising a steroid responsive promoter, the promoter consisting essentially of a plurality of glucocorticoid response elements (GREs), a TATA box, and an initiator element containing a transcriptional initiator site located from 20 to 50 bases from the TATA box, the promoter lacking upstream elements which bind nuclear factor I, and the vector further comprising a restriction endonuclease site downstream from the promoter for insertion of DNA to be expressed from the promoter, wherein the DNA is expressed from the vector in an animal cell.
Claims10
An expression vector adapted for replication in an animal cell comprising a glucocorticoid responsive promoter, said promoter comprising a plurality of at least 5 glucocorticoid response elements (GREs), a viral or mammalian TATA box, and a viral or mammalian initiator element with a transcriptional initiator site located from 20 to 50 bases from said TATA box, said promoter lacking upstream elements which bind nuclear factor I, and said vector further comprising a restriction endonuclease site downstream from said promoter for insertion of DNA to be expressed from said promoter;
- wherein said DNA is expressed from said vector in an animal cell.
A promoter consisting of a plurality of at least five glucocorticoid response elements (GREs), a TATA box, and an initiator site containing a transcriptional initiator site located from 20 to 50 bases from said TATA box, said promoter lacking upstream elements which bind nuclear factor I, wherein said promoter is responsive to ligand-bound glucocorticoid, progesterone, androgen or mineralocorticoid receptor when transiently transfected into cells, when stably integrated within a genome, or when stably propagated in an episomal vector.
Dependencies are inferred from the claim text.